Fragmentation study of five trichothecenes using electrospray hybrid ion trap/time-of-flight mass spectrometry with accurate mass measurements

被引:16
作者
Liu, Zhao-Ying [1 ]
Yu, Chun-Hong [1 ]
Wan, Leren [2 ]
Sun, Zhi-Liang [1 ]
机构
[1] Hunan Agr Univ, Coll Vet Med, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Biol Vet Drugs Branch, Changsha 410128, Hunan, Peoples R China
[2] Shimadzu Int Trading Shanghai Co Ltd, Shanghai 200052, Peoples R China
关键词
Trichothecenes; Ion trap/time-of-flight mass spectrometry; Accurate mass; Fragmentation; B-TRICHOTHECENE; IONIZATION; CEREALS;
D O I
10.1016/j.ijms.2011.09.007
中图分类号
O64 [物理化学(理论化学)、化学物理学]; O56 [分子物理学、原子物理学];
学科分类号
070203 ; 070304 ; 081704 ; 1406 ;
摘要
Five trichothecenes, T-2 toxin, HT-2 toxin, deoxynivalenol (DON), 3-acetyl-DON (3-AcDON) and nivalenol (NIV), major class of mycotoxins produced by Fusarium, were investigated by electrospray hybrid ion trap/time-of-flight mass spectrometry (IT-TOF-MS). Fragmentation mechanisms are proposed based on the MS(2) and MS(3) experiments and accurate mass measurements. The sodium adduct ions [M+Na](+). of type-A trichothecenes, such as T-2 toxin and HT-2 toxin, were observed in the MS spectra. However, the [M-H](-) ions of type-B trichothecenes, such as NIV, and 3-AcDON, were observed in the MS spectra. Eliminations of isovaleryloxy group and acetic acid were the common fragmentation pathways of T-2 toxin and HT-2 toxin. However, the cleavage of epoxy was the common fragmentation pathways of DON, 3-AcDON and NIV. The other fragmentation pathways were found to be dependent on the substitutent group. The data reported here may help to identify analogues of the trichothecenes that are commonly co-produced by Fusarium in cultures or naturally contaminated samples. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:133 / 140
页数:8
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