Quick method for grouper species identification using real-time PCR

被引：14

作者：

Chen, Shuangya ^{[1
]}

Zhang, Jin ^{[2
]}

Chen, Weiling ^{[1
]}

Zhang, Yongxiang ^{[1
]}

Wang, Jiahe ^{[1
]}

Xu, Dunming ^{[1
]}

Zhou, Yu ^{[1
]}

机构：

[1] Xiamen Entry Exit Inspect & Quarantine Technol Ct, Xiamen 361026, Fujian, Peoples R China

[2] Agilent Technol Co Ltd, Beijing 100102, Peoples R China

来源：

FOOD CONTROL | 2012年 / 27卷 / 01期

关键词：

Real-time PCR; Grouper; Species identification; COMMERCIAL PRODUCTS; QUANTIFICATION; AUTHENTICATION;

D O I：

10.1016/j.foodcont.2012.03.011

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A convenient and quick method based on real-time polymerase chain reaction (PCR) was established to identify 10 grouper species in China. According to the sequence of mitochondrial cytochrome oxidase subunit I (COI) gene, primers and probe were designed. PCR amplifications of 129 bp of COI gene sequences from 10 grouper species of four genera (Epinephelus, Promicrops, Plectropomus and Cromileptes) were compared with that of 30 other fish species. PCR amplifications were only observed in 10 grouper species. The result suggested that real-time PCR can be a powerful tool for grouper species diagnoses. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.

引用

页码：108 / 112

页数：5

共 9 条

[1] Species identification of grouper and snapper in Taiwan Strait using polymerase chain reaction-restriction fragment length polymorphism analysis and lab-on-a-chip system [J].

Chen Shuangya ;

Zhang Jin ;

Chen Weiling ;

Xu Dunming ;

Zhou Yu .

CHINESE JOURNAL OF CHROMATOGRAPHY, 2011, 29 (07) :677-680

[2] Interlaboratory Evaluation of a Real-Time Multiplex Polymerase Chain Reaction Method for Identification of Salmon and Trout Species in Commercial Products [J].

Hellberg, Rosalee S. Rasmussen ;

Naaum, Amanda M. ;

Handy, Sara M. ;

Hanner, Robert H. ;

Deeds, Jonathan R. ;

Yancy, Haile F. ;

Morrissey, Michael T. .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2011, 59 (03) :876-884

[3] Authentication of Atlantic Cod (Gadus morhua) Using Real Time PCR [J].

Herrero, Beatriz ;

Madrinan, Maria ;

Vieites, Juan M. ;

Espineira, Montserrat .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (08) :4794-4799

[4] Development of a method for the quantification of haddock (Melanogrammus aeglefinus) in commercial products using real-time PCR [J].

Hird, HJ ;

Hold, GL ;

Chisholm, J ;

Reece, P ;

Russell, VJ ;

Brown, J ;

Goodier, R ;

MacArthur, R .

EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2005, 220 (5-6) :633-637

[5] Application of relative quantification TaqMan real-time polymerase chain reaction technology for the identification and quantification of Thunnus alalunga and Thunnus albacares [J].

Lopez, I ;

Pardo, MA .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (11) :4554-4560

[6] Authentication of flying-fish-meal content of processed food using PCR-RFLP [J].

Nagase, Mitsutoshi ;

Maeta, Kazuhiko ;

Aimi, Tadanori ;

Suginaka, Katsuaki ;

Morinaga, Tsutomu .

FISHERIES SCIENCE, 2009, 75 (03) :811-816

[7]

Palumbi Stephen R., 1996, P205

[8] Identification of European Hake Species (Merluccius merluccius) Using Real-Time PCR [J].

Sanchez, A. ;

Quinteiro, J. ;

Rey-Mendez, M. ;

Perez-Martin, R. I. ;

Sotelo, C. G. .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (09) :3397-3403

[9] Multiplex PCR method for use in real-time PCR for identification of fish fillets from grouper (Epinephelus and Mycteroperca species) and common substitute species [J].

Trotta, M ;

Schönhuth, S ;

Pepe, T ;

Cortesi, ML ;

Puyet, A ;

Bautista, JM .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (06) :2039-2045

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