Molecular and functional analysis indicates a mosaic structure of Salmonella pathogenicity island 2

被引：69
作者：
Hensel, M [1 ]
Nikolaus, T [1 ]
Egelseer, C [1 ]
机构：
[1] Max Von Pettenkofer Inst Hyg & Med Microbiol, Lehrstuhl Bakteriol, D-80336 Munich, Germany
来源：
MOLECULAR MICROBIOLOGY | 1999年 / 31卷 / 02期
关键词：
D O I：
10.1046/j.1365-2958.1999.01190.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two large virulence loci encoding type III secretion systems are present on the chromosome of Salmonella typhimurium. Salmonella pathogenicity island 2 (SPI2) is important for the survival of S. typhimurium in host organs and forms an insertion of about 40 kb at the tRNA(Val) gene. However, several indications suggested that SPI2 was not the result of a single event of horizontal gene transfer. We characterized the portion of SPI2 towards the 30 cs boundary and performed mutational analysis to investigate the contribution of this region to S. enterica virulence. This analysis indicates that SPI2 may be composed of at least two different genetic elements. About 15 kb of the 40 kb of SPI2 contain genes without a significant contribution to systemic infections in the model of murine salmonellosis. Our study allowed us to define genes in SPI2 important for virulence further and indicated that this locus has a complex mosaic structure.
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页码：489 / 498
页数：10
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