Osteogenic Effect of Rabbit Periosteum-Derived Precursor Cells Co-Induced by Electric Stimulation and Adipose-Derived Stem Cells in a 3D Co-Culture System

被引：0
作者：
Chen, Alvin Chao-Yu [1 ,2 ]
Tong, Yun-Wen [3 ]
Chiu, Chih-Hao [2 ,4 ,5 ]
Lei, Kin Fong [6 ,7 ]
机构：
[1] Chang Gung Mem Hosp, Bone & Joint Res Ctr, Dept Orthoped Surg, Linkou 333, Taiwan
[2] Chang Gung Mem Hosp, Comprehens Sports Med Ctr, Linkou 333, Taiwan
[3] Chang Gung Mem Hosp, Dept Orthoped Surg, Linkou 333, Taiwan
[4] Chang Gung Mem Hosp, Dept Orthoped Surg, Taoyuan 333, Taiwan
[5] Chang Gung Mem Hosp, Bone & Joint Res Ctr, Linkou 333, Taiwan
[6] Chang Gung Univ, Grad Inst Biomed Engn, Taoyuan 333, Taiwan
[7] Chang Gung Mem Hosp, Dept Radiat Oncol, Linkou 333, Taiwan
来源：
IEEE OPEN JOURNAL OF NANOTECHNOLOGY | 2021年 / 2卷
关键词：
Three-dimensional displays; Bones; Substrates; Stem cells; Hospitals; Rabbits; Nanotechnology; Periosteum-derived progenitor cells; adipose-derived stem cells; osteogenesis; 3D Co-culture; electric stimulation; orthopedics; rehabilitation; BONE-MARROW; DIFFERENT STIFFNESS; IN-VIVO; TISSUE; DIFFERENTIATION; OSTEOPONTIN; MICROSTRUCTURE; SCAFFOLDS; PATHWAY; FIELDS;
D O I：
10.1109/OJNANO.2021.3131653
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
Periosteum-derived progenitor cells (PDPCs) are highly promising cell sources for bone fracture healing because of their stem cell-like multipotency to undergo osteogenesis and chondrogenesis. Both externally physical stimulation and internally biochemical signal were reported to enhance osteogenic differentiation of bone tissues. Electric stimulation (ES) could trigger the differentiation of stem cells, like mesenchymal stem cells (MSCs) and adipose-derived stem cells (ADSCs). But the effect is still unclear on PDPCs. In order to investigate the differentiation ability of PDPCs co-induced by ES and ADSCs, a biomimetic 3-dimensional (3D) co-culture system was developed for providing ES and co-culturing with ADSCs. Gene expression was studied after a 3-day culture course. From our results, osteogenic differentiation of PDPCs was significantly activated under the ES of 0.7 V/cm, 80 kHz, and 3 hrs/day. Moreover, co-culturing with ADSCs during the ES treatment was found to have synergistic effect of osteogenic differentiation. In addition, chondrogenic differentiation was shown when the PDPCs were cultured for a long culture course. In summary, osteogenic differentiation of PDPCs was shown to be co-induced by ES and ADSCs. This study provides significant insights of the PDPC therapy for bone tissue regeneration.
引用
收藏
页码：153 / 160
页数：8
相关论文
共 56 条
[11]   Concise Review: The Periosteum: Tapping into a Reservoir of Clinically Useful Progenitor Cells [J].
Chang, Hana ;
Tate, Melissa L. Knothe .
STEM CELLS TRANSLATIONAL MEDICINE, 2012, 1 (06) :480-491
[12]   Co-culturing mesenchymal stem cells from bone marrow and periosteum enhances osteogenesis and neovascularization of tissue-engineered bone [J].
Chen, Daoyun ;
Zhang, Xianlong ;
He, Yaohua ;
Lu, Jianxi ;
Shen, Hao ;
Jiang, Yao ;
Zhang, Changqing ;
Zeng, Bingfang .
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2012, 6 (10) :822-832
[13]   Cell-free scaffolds with different stiffness but same microstructure promote bone regeneration in rabbit large bone defect model [J].
Chen, Guobao ;
Yang, Li ;
Lv, Yonggang .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2016, 104 (04) :833-841
[14]   3D Scaffolds with Different Stiffness but the Same Microstructure for Bone Tissue Engineering [J].
Chen, Guobao ;
Dong, Chanjuan ;
Yang, Li ;
Lv, Yonggang .
ACS APPLIED MATERIALS & INTERFACES, 2015, 7 (29) :15790-15802
[15]  
Colnot C, 2009, J BONE MINER RES, V24, P274, DOI [10.1359/jbmr.081003, 10.1359/JBMR.081003]
[16]   EFFECT OF ELECTRICAL-STIMULATION ON BIOPHYSICAL PROPERTIES OF FRACTURE HEALING [J].
CONNOLLY, JF ;
ORTIZ, J ;
PRICE, RR ;
BAYUZICK, RJ .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1974, 238 (OCT11) :519-529
[17]   Co-culture of canine mesenchymal stem cells with primary bone-derived osteoblasts promotes osteogenic differentiation [J].
Csaki, C. ;
Matis, U. ;
Mobasheri, A. ;
Shakibaei, M. .
HISTOCHEMISTRY AND CELL BIOLOGY, 2009, 131 (02) :251-266
[18]   Mesenchymal multipotency of adult human periosteal cells demonstrated by single-cell lineage analysis [J].
De Bari, C ;
Dell'Accio, F ;
Vanlauwe, J ;
Eyckmans, J ;
Khan, YM ;
Archer, CW ;
Jones, EA ;
McGonagle, D ;
Mitsiadis, TA ;
Pitzalis, C ;
Luyten, FP .
ARTHRITIS AND RHEUMATISM, 2006, 54 (04) :1209-1221
[19]   The periosteum: what is it, where is it, and what mimics it in its absence? [J].
Dwek, Jerry R. .
SKELETAL RADIOLOGY, 2010, 39 (04) :319-323
[20]   ENHANCEMENT OF FRACTURE-HEALING [J].
EINHORN, TA .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1995, 77A (06) :940-956
123456