Fluorescence generation from tandem repeats of a malachite green RNA aptamer using rolling circle transcription

被引：12

作者：

Furukawa, Kazuhiro ^{[1
,2
]}

Abe, Hiroshi ^{[1
]}

Abe, Naoko ^{[1
]}

Harada, Mitsuru ^{[1
,2
]}

Tsuneda, Satoshi ^{[2
]}

Ito, Yoshihiro ^{[1
]}

机构：

[1] RIKEN, Nano Med Engn Lab, Adv Sci Inst, Wako, Saitama 3510198, Japan

[2] Waseda Univ, Dept Life Sci & Med Biosci, Shinjuku Ku, Tokyo 1698555, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2008年 / 18卷 / 16期

关键词：

fluorescence; nucleic acid; amplification; RNA; polymerase;

D O I：

10.1016/j.bmcl.2008.07.040

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

We demonstrate a generation of tandem repeats of a malachite green (MG) RNA aptamer using rolling circle transcription. To keep the higher-order structure of each aptamer on long RNA, we designed a sequence of circular DNA with a 14-base linker. T7 RNA polymerase was superior to Escherichia coli RNA polymerase in the specific transcription of the MG RNA aptamer. Finally. the generation of the fluorescence signal was confirmed from aptamer repeats with MG. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：4562 / 4565

页数：4

共 17 条

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