Triterpenoid Constituents from the Roots of Paeonia rockii ssp rockii

被引：34

作者：

Mencherini, Teresa ^{[1
]}

Picerno, Patrizia ^{[1
]}

Festa, Michela ^{[1
]}

Russo, Paola ^{[1
]}

Capasso, Anna ^{[1
]}

Aquino, Rita ^{[1
]}

机构：

[1] Univ Salerno, Dipartimento Sci Farmaceut & Biomed, I-84084 Fisciano, SA, Italy

来源：

JOURNAL OF NATURAL PRODUCTS | 2011年 / 74卷 / 10期

关键词：

BETULINIC ACID; CELL-LINES; APOPTOSIS; SAPONINS; MELANOMA; CULTURES; RADIX; P53;

D O I：

10.1021/np200359v

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

An investigation of a chloroform-soluble extract from the roots of Paeonia rockii ssp. rockii yielded three new noroleanane triterpenoids (1-3) together with 19 known compounds. Their structures were established by analysis of the spectroscopic data. The effects of this chloroform-soluble extract and its major constituents on cell proliferation and apoptosis of a panel of human cancer cell lines (melanoma M-14, colon cancer HT-29, breast cancer MCF-7) were evaluated by the MTT bioassay and propidium iodide staining, respectively, in comparison with normal human embryonic kidney cells (HEK-293). Two of the triterpenoids, betulinic acid (4) and oleanolic acid (5), and the crude extract were cytotoxic and induced apoptosis selectively in the M-14 melanoma cell line. This effect was reversed by the caspase-inhibitor z-VAD-fmk, suggesting that such action is mediated by caspase-3 activation.

引用

页码：2116 / 2121

页数：6

共 37 条

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