Construction of a Lytically Replicating Kaposi's Sarcoma-Associated Herpesvirus

被引：25

作者：

Budt, Matthias ^{[2
]}

Hristozova, Tsvetana ^{[2
]}

Hille, Georg ^{[2
]}

Berger, Katrin ^{[2
]}

Brune, Wolfram ^{[1
,2
]}

机构：

[1] Heinrich Pette Inst, Leibniz Inst Expt Virol, D-20251 Hamburg, Germany

[2] Robert Koch Inst, Div Viral Infect, D-13353 Berlin, Germany

来源：

JOURNAL OF VIROLOGY | 2011年 / 85卷 / 19期

关键词：

BACTERIAL ARTIFICIAL CHROMOSOME; PRIMARY EFFUSION LYMPHOMA; ENDOTHELIAL-CELLS; ANTIVIRAL DRUGS; INFECTION; PROTEIN; GENE; EXPRESSION; IDENTIFICATION; DISRUPTION;

D O I：

10.1128/JVI.05071-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Karposi's sarcoma-associated herpesvirus (KSHV) is found predominantly in a latent state in most cell types, impeding investigations of the lytic replication cycle. Here, we engineered the cloned KSHV genome, bacterial artificial chromosome 36 (BAC36), to enforce constitutive expression of the main lytic switch regulator, the replication and transcription activator (RTA) (open reading frame 50 [ORF50]). The resulting virus, KSHV-lyt, activated by default the lytic cycle and replicated to high titers in various cells. Using KSHV-lyt, we showed that ORF33 (encoding a tegument protein) is essential for lytic KSHV replication in cell culture, but ORF73 (encoding the latent nuclear antigen [LANA]) is not. Thus, KSHV-lyt should be highly useful to study viral gene function during lytic replication.

引用

收藏

页码：10415 / 10420

页数：6

相关论文

共 27 条

[11] Induction of apoptosis limits cytomegalovirus cross-species infection [J].

Jurak, Igor ;

Brune, Wolfram .

EMBO JOURNAL, 2006, 25 (11) :2634-2642

[12] Sensitivity of Kaposi's sarcoma-associated herpesvirus replication to antiviral drugs - Implications for potential therapy [J].

Kedes, DH ;

Ganem, D .

JOURNAL OF CLINICAL INVESTIGATION, 1997, 99 (09) :2082-2086

[13] De novo infection and serial transmission of Kaposi's sarcoma-associated herpesvirus in cultured endothelial cells [J].

Lagunoff, M ;

Bechtel, J ;

Venetsanakos, E ;

Roy, AM ;

Abbey, N ;

Herndier, B ;

McMahon, M ;

Ganem, D .

JOURNAL OF VIROLOGY, 2002, 76 (05) :2440-2448

[14] Genetic disruption of KSHV major latent nuclear antigen LANA enhances viral lytic transcriptional program [J].

Li, Qiuhua ;

Zhou, Fuchun ;

Ye, Fengchun ;

Gao, Shou-Jiang .

VIROLOGY, 2008, 379 (02) :234-244

[15] Transcriptional activation by the product of open reading frame 50 of Kaposi's sarcoma-associated herpesvirus is required for lytic viral reactivation in B cells [J].

Lukac, DM ;

Kirshner, JR ;

Ganem, D .

JOURNAL OF VIROLOGY, 1999, 73 (11) :9348-9361

[16]

Mahy B. W. J., 1996, Virology Methods Manual

[17] Oral ganciclovir for patients with cytomegalovirus retinitis treated with a ganciclovir implant [J].

Martin, DF ;

Kuppermann, BD ;

Wolitz, RA ;

Palestine, AG ;

Li, H ;

Robinson, CA .

NEW ENGLAND JOURNAL OF MEDICINE, 1999, 340 (14) :1063-1070

[18] The full-length protein encoded by human cytomegalovirus gene UL117 is required for the proper maturation of viral replication compartments [J].

Qian, Zhikang ;

Xuan, Baoqin ;

Hong, Te Tee ;

Yu, Dong .

JOURNAL OF VIROLOGY, 2008, 82 (07) :3452-3465

[19] A self-recombining bacterial artificial chromosome and its application for analysis of herpesvirus pathogenesis [J].

Smith, GA ;

Enquist, LW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (09) :4873-4878

[20] A viral gene that activates lytic cycle expression of Kaposi's sarcoma-associated herpesvirus [J].

Sun, R ;

Lin, SF ;

Gradoville, L ;

Yuan, Y ;

Zhu, FX ;

Miller, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (18) :10866-10871