Mutant DNA Detection on Graphene Oxide with SYBR Green I

被引：5

作者：

Lee, Chunghyun ^{[1
]}

Gang, Jongback ^{[1
]}

机构：

[1] Gachon Univ, Dept Nano Chem, Sungnam 461701, Kyunggido, South Korea

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2016年 / 16卷 / 11期

关键词：

Graphene Oxide; DNA Adsorption; Mutation Detection; Reaction Condition; Mismatched DNA; SYBR Green I; MUTATION DETECTION; GRADIENT ELECTROPHORESIS; STRANDED-DNA; AMPLIFICATION;

D O I：

10.1166/jnn.2016.13609

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Graphene oxide (GO) has a large surface-to-volume ratio and contains hydrophobic hexagonal rings that can interact with biomolecules. Single-stranded DNA (ssDNA) adsorbs more strongly to the surface of GO than double stranded DNA (dsDNA) via hydrophobic interaction between nucleobases of DNA and the hydrophobic surface of GO. This study was designed to detect samples containing various mismatched DNAs under different pH and salt conditions by sequential use of GO and SYBR Green I. Our results demonstrated that a GO-based fluorescence assay showed little difference in fluorescence intensity between perfectly matched DNA (pmDNA) and mutant DNAs. However, an SYBR Green I-assisted assay elicited large difference in fluorescence intensity between the pmDNA and mismatched DNAs. This enhances the detection sensitivity and efficiency between pmDNA and mutant DNA.

引用

页码：11868 / 11872

页数：5

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