A novel method for the measurement of oxygen mass transfer rates in small-scale vessels

被引：18
|
作者：
Ortiz-Ochoa, K
Doig, SD
Ward, JM
Baganz, F
机构：
[1] UCL, Adv Ctr Biochem Engn, Dept Biochem Engn, London WC1E 7JE, England
[2] UCL, Dept Biochem & Mol Biol, London WC1E 6BT, England
来源：
BIOCHEMICAL ENGINEERING JOURNAL | 2005年 / 25卷 / 01期
基金：
英国工程与自然科学研究理事会;
关键词：
enzyme kinetics; bio-oxidation; mass transfer; small-scale;
D O I：
10.1016/j.bej.2005.04.003
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A novel method for the measurement of the volumetric oxygen transfer coefficient, k(L)a, using the catechol-2,3-dioxygenase (XyIE) bio-oxidation of catechol yielding 2-hydroxymuconic semialdehyde (2-HS), has been developed for small-scale systems. This method was kinetically characterized and validated by comparison to other established techniques. The bio-oxidation rate was found to be zero order over a catechol concentration from 2 to 7 mM l(-1). When the enzyme concentration was in excess and the bio-oxidation rate was mass transfer limited, indicated by a percent DOT of zero, the oxygen transfer rate was determined from the linear increase in product concentration. The method was validated in a 21 stirred tank vessel equipped with a DOT probe and connected to a gas mass spectrometer. The novel method yielded similar kLa values when compared to the dynamic gassing out method under the same conditions. The applicability of this novel method for small-scale devices was demonstrated by measuring kLa values of up to 150 h(-1) in shaken microplates with a working volume of only 200 mu l. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：63 / 68
页数：6
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