A multiplex RT-PCR assay for detection of emergent pepper Tsw resistance-breaking variants of tomato spotted wilt virus in South Korea

被引：4

作者：

Kwon, Sun-Jung ^{[1
]}

Cho, Young-Eun ^{[1
]}

Byun, Hee-Seong ^{[2
]}

Kwak, Hae-Ryun ^{[2
]}

Seo, Jang-Kyun ^{[1
,3
]}

机构：

[1] Seoul Natl Univ, Inst Green Bio Sci, Pyeongchang 25354, South Korea

[2] Rural Dev Adm, Natl Inst Agr Sci, Crop Protect Div, Wonju 55365, South Korea

[3] Seoul Natl Univ, Inst Green Bio Sci, Dept Int Agr Technol, Pyeongchang 25354, South Korea

来源：

MOLECULAR AND CELLULAR PROBES | 2022年 / 61卷

关键词：

Tomato spotted wilt virus; Resistance-breaking; Molecular diagnosis; Multiplex RT-PCR; Pepper; NSS PROTEIN; CAPSICUM-CHINENSE; GENE; STRAINS; AVIRULENCE; DIVERSITY; OVERCOME; THRIPS; PLANT;

D O I：

10.1016/j.mcp.2022.101792

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tomato spotted wilt virus (TSWV) is a highly destructive virus for pepper. Introgression of the resistance gene Tsw in pepper is used to manage TSWV worldwide; however, the occurrence of Tsw resistance-breaking (RB) variants threatens the pepper industry. Here, we developed a multiplex reverse-transcription PCR assay for detection of recently emerged Tsw RB variants in South Korea with high specificity and sensitivity.

引用

页数：5

共 32 条

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