A multiplex RT-PCR assay for detection of emergent pepper Tsw resistance-breaking variants of tomato spotted wilt virus in South Korea

被引：4
作者：
Kwon, Sun-Jung [1 ]
Cho, Young-Eun [1 ]
Byun, Hee-Seong [2 ]
Kwak, Hae-Ryun [2 ]
Seo, Jang-Kyun [1 ,3 ]
机构：
[1] Seoul Natl Univ, Inst Green Bio Sci, Pyeongchang 25354, South Korea
[2] Rural Dev Adm, Natl Inst Agr Sci, Crop Protect Div, Wonju 55365, South Korea
[3] Seoul Natl Univ, Inst Green Bio Sci, Dept Int Agr Technol, Pyeongchang 25354, South Korea
来源：
MOLECULAR AND CELLULAR PROBES | 2022年 / 61卷
关键词：
Tomato spotted wilt virus; Resistance-breaking; Molecular diagnosis; Multiplex RT-PCR; Pepper; NSS PROTEIN; CAPSICUM-CHINENSE; GENE; STRAINS; AVIRULENCE; DIVERSITY; OVERCOME; THRIPS; PLANT;
D O I：
10.1016/j.mcp.2022.101792
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Tomato spotted wilt virus (TSWV) is a highly destructive virus for pepper. Introgression of the resistance gene Tsw in pepper is used to manage TSWV worldwide; however, the occurrence of Tsw resistance-breaking (RB) variants threatens the pepper industry. Here, we developed a multiplex reverse-transcription PCR assay for detection of recently emerged Tsw RB variants in South Korea with high specificity and sensitivity.
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页数：5
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