Analysis and characterization of sophorolipids by liquid chromatography with atmospheric pressure chemical ionization

被引：74

作者：

Nuñez, A ^{[1
]}

Ashby, R ^{[1
]}

Foglia, TA ^{[1
]}

Solaiman, DKY ^{[1
]}

机构：

[1] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA

来源：

CHROMATOGRAPHIA | 2001年 / 53卷 / 11-12期

关键词：

column liquid chromatography; liquid chromatography-mass spectrometry; atmospheric pressure chemical ionization; Candida bombicola; sophorolipids;

D O I：

10.1007/BF02493019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed phase high performance liquid chromatographic method combined with atmospheric pressure chemical ionization mass detection (LC/APCI-MS) has been developed for the separation and analysis of sophorolipids produced by C. bombicola when grown on fatty acid mixtures. Using this method it was found that the incorporation of palmitic, linoleic, and linolenic acids into the sophorolipid structure was dependent on the initial fatty acid content of these acids, whereas the incorporation of oleic acid was independent of its initial content in the mixture. Also observed was the incorporation of esterified glycerol into the sophorolipid structure, which has not been reported previously.

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页码：673 / 677

页数：5

相关论文

共 10 条

[1] MICROBIAL-PRODUCTION, STRUCTURE ELUCIDATION AND BIOCONVERSION OF SOPHOROSE LIPIDS [J].

ASMER, HJ ;

LANG, S ;

WAGNER, F ;

WRAY, V .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1988, 65 (09) :1460-1466

[2] Enzyme-mediated regioselective acylations of sophorolipids [J].

Bisht, KS ;

Gross, RA ;

Kaplan, DL .

JOURNAL OF ORGANIC CHEMISTRY, 1999, 64 (03) :780-789

[3] Sophorolipid production by Candida bombicola:: Medium composition and culture methods [J].

Casas, JA ;

García-Ochoa, F .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 88 (05) :488-494

[4] Production of sophorolipids in high concentration from deproteinized whey and rapeseed oil in a two stage fed batch process using Candida bombicola ATCC 22214 and Cryptococcus curvatus ATCC 20509 [J].

Daniel, HJ ;

Reuss, M ;

Syldatk, C .

BIOTECHNOLOGY LETTERS, 1998, 20 (12) :1153-1156

[5] SOPHOROSE LIPID PRODUCTION FROM LIPIDIC PRECURSORS - PREDICTIVE EVALUATION OF INDUSTRIAL SUBSTRATES [J].

DAVILA, AM ;

MARCHAL, R ;

VANDECASTEELE, JP .

JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1994, 13 (04) :249-257

[6]

Haverkamp J, 1995, ANAL BIOCHEM, V230, P135

[7] High-performance liquid chromatography method for the characterization of rhamnolipid mixtures produced by Pseudomonas aeruginosa UG2 on corn oil [J].

Mata-Sandoval, JC ;

Karns, J ;

Torrents, A .

JOURNAL OF CHROMATOGRAPHY A, 1999, 864 (02) :211-220

[8] Production of sophorolipids from whey II.: Product composition, surface active properties, cytotoxicity and stability against hydrolases by enzymatic treatment [J].

Otto, RT ;

Daniel, HJ ;

Pekin, G ;

Müller-Decker, K ;

Fürstenberger, G ;

Reuss, M ;

Syldatk, C .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1999, 52 (04) :495-501

[9] High- and low-molecular-mass microbial surfactants [J].

Rosenberg, E ;

Ron, EZ .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1999, 52 (02) :154-162

[10] FERMENTATION OF LONG-CHAIN COMPOUNDS BY TORULOPSIS MAGNOLIAE .1. STRUCTURES OF HYDROXY FATTY ACIDS OBTAINED BY FERMENTATION OF FATTY ACIDS AND HYDROCARBONS [J].

TULLOCH, AP ;

SPENCER, JFT ;

GORIN, PAJ .

CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE, 1962, 40 (07) :1326-&