The Immune Response to the RT181-189 Epitope in HIV-1-Infected Patients is Associated with Viral Sequence Polymorphism Flanking the Epitope

被引：2

作者：

Pacheco, Yovana ^{[2
]}

Allavena, Clotilde ^{[2
,3
]}

Guilloux, Yannick ^{[4
,7
]}

Mueller-Schmucker, Sandra M. ^{[5
]}

Hueckelhoven, Angela G. ^{[5
]}

Andre-Garnier, Elisabeth ^{[2
,6
]}

Cleon, Francois ^{[2
]}

Ferre, Virginie ^{[2
,6
]}

Rodallec, Audrey ^{[6
]}

Billaud, Eric ^{[3
]}

Harrer, Thomas ^{[5
]}

Raffi, Francois ^{[2
,3
]}

McIlroy, Dorian ^{[1
,2
,7
]}

机构：

[1] EA4271 Fac Med & Pharm, F-44035 Nantes, France

[2] Univ Nantes, Lab Immunovirol & Polymorphisme Genet EA4271, Nantes, France

[3] Hop Hotel Dieu, Serv Infectiol, Nantes, France

[4] INSERM, UMR 892, Nantes, France

[5] Univ Erlangen Nurnberg, Univ Hosp Erlangen, Dept Internal Med 3, D-91054 Erlangen, Germany

[6] Hop Hotel Dieu, Virol Lab, Nantes, France

[7] Univ Nantes, Fac Sci & Tech, Nantes, France

来源：

JOURNAL OF CLINICAL IMMUNOLOGY | 2011年 / 31卷 / 04期

关键词：

Cytotoxic T lymphocytes; T-cell epitope; HIV-1 reverse transcriptase; antiviral drug resistance; IMMUNODEFICIENCY-VIRUS TYPE-1; CYTOTOXIC T-LYMPHOCYTES; ANTIRETROVIRAL THERAPY; REVERSE-TRANSCRIPTASE; CELL RESPONSES; HIV-1; INFECTION; RECOGNITION; GAG; VIREMIA; ESCAPE;

D O I：

10.1007/s10875-011-9520-z

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Many drug-resistance mutations in HIV-1 reverse transcriptase fall within cytotoxic T lymphocytes (CTL) epitopes, but studies of the response to these epitopes in patients with virological failure are lacking. We therefore compared IFN-gamma ELISPOT responses to the YV9 epitope (RT181-189) covering the lamivudine resistance mutation, M184V, in HLA-A2(+) antiretroviral treatment (ART)-naive patients (n = 19), to those found in HLA-A2(+) patients with virological failure (n = 15). Ten ART-naive patients had an ELISPOT response to the wild-type epitope that cross-reacted with the mutant epitope. Two patients with virological failure showed a specific response to the 184V mutant epitope. Responses against YV9 were strongly associated (p = 0.005) with the presence of a 177E mutation, and the same tendency was observed in an independent cohort of patients (n = 22). These results indicate that variants in flanking residues may influence CTL responses to conserved subdominant HIV-1 epitopes.

引用

页码：681 / 689

页数：9

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