Analysis of apoptosis regulatory genes expression in the bone marrow (BM) of adult de novo Myelodysplastic Syndromes (MDS)

被引：13

作者：

Economopoulou, C. ^{[1
]}

Pappa, V. ^{[1
]}

Kontsioti, F. ^{[1
]}

Papageorgiou, S. ^{[1
]}

Kapsimali, V. ^{[1
]}

Papasteriadi, C. ^{[1
]}

Economopoulou, P. ^{[1
]}

Papageorgiou, E. ^{[1
]}

Dervenoulas, J. ^{[1
]}

Economopoulos, T. ^{[1
]}

机构：

[1] Attikon Univ Hosp, Propedeut Clin Internal 2, Athens, Haidari, Greece

来源：

LEUKEMIA RESEARCH | 2008年 / 32卷 / 01期

关键词：

MDS; apoptosis; Annexin V; RNAse protection system; bcl-2; caspases;

D O I：

10.1016/j.leukres.2007.04.012

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The aim of the present Study was to examine caspases, granzyme B and bcl-2 family mRNA expression and the degree of apoptosis in the bone marrow (BM) of 46 Myelodysplastic Syndromes (MDS) and to correlate our findings with clinical parameters. The degree of apoptosis was determined by Annexin V, whereas expression of genes was determined using a multiprobe RNase Protection System. A positive correlation was found between caspases 8, 5, 3, 2, 1 and the level of apoptosis. bfl 1 and mcl 1 levels were significantly higher in patients with BM blasts > 5%. Cases with ratio of bid expression > 1 compared to normal pool were associated with IPSS values <= 1. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：61 / 69

页数：9

共 42 条

[1] Sequential activation of caspase-1 and caspase-3-like proteases during apoptosis in myelodysplastic syndromes [J].

Ali, A ;

Mundle, SD ;

Ragasa, D ;

Reza, S ;

Shetty, V ;

Mativi, BY ;

Cartlidge, JD ;

Azharuddin, M ;

Qawi, H ;

Dar, S ;

Raza, A .

JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH, 1999, 8 (04) :343-356

[2] Measurement of spontaneous and therapeutic agent-induced apoptosis with BCL-2 protein expression in acute myeloid leukemia [J].

Banker, DE ;

Groudine, M ;

Norwood, T ;

Appelbaum, FR .

BLOOD, 1997, 89 (01) :243-255

[3] Incidence and role of apoptosis in myelodysplastic syndrome: morphological and ultrastructural assessment [J].

Bogdanovic, AD ;

Trpinac, DP ;

Jankovic, GM ;

Bumbasirevic, VZ ;

Obradovic, M ;

Colovic, MD .

LEUKEMIA, 1997, 11 (05) :656-659

[4] Expression and activity of caspases 1 and 3 in myelodysplastic syndromes [J].

Boudard, D ;

Sordet, O ;

Vasselon, C ;

Revol, V ;

Berthéas, MF ;

Freyssenet, D ;

Viallet, A ;

Piselli, S ;

Guyotat, D ;

Campos, L .

LEUKEMIA, 2000, 14 (12) :2045-2051

[5] Activity of the caspase-3/CPP32 enzyme is increased in "early stage" myelodysplastic syndromes with excessive apoptosis, but caspase inhibition does not enhance colony formation in vitro [J].

Bouscary, D ;

Chen, YL ;

Guesnu, M ;

Picard, F ;

Viguier, F ;

Lacombe, C ;

Dreyfus, F ;

Fontenay-Roupie, M .

EXPERIMENTAL HEMATOLOGY, 2000, 28 (07) :784-791

[6] COMPARATIVE QUANTITATIVE EXPRESSION OF BCL-2 BY NORMAL AND LEUKEMIC MYELOID CELLS [J].

BRADBURY, DA ;

RUSSELL, NH .

BRITISH JOURNAL OF HAEMATOLOGY, 1995, 91 (02) :374-379

[7]

CAMPOS L, 1993, BLOOD, V81, P3091

[8]

Chen Jia-lin, 2003, Zhonghua Xue Ye Xue Za Zhi, V24, P565

[9] Apoptotic rate varies with detection methods in myelodysplastic syndromes: impact of method selection [J].

Choi, JW .

EUROPEAN JOURNAL OF HAEMATOLOGY, 2002, 69 (01) :62-63

[10]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

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