Insulin and IGF-I receptors signaling in protection from apoptosis

被引:99
作者
Prisco, M [1 ]
Romano, G [1 ]
Peruzzi, F [1 ]
Valentinis, B [1 ]
Baserga, R [1 ]
机构
[1] Thomas Jefferson Univ, Kimmel Canc Ctr, Philadelphia, PA 19107 USA
关键词
IGF-I; insulin-like growth factor; IGF-IR; IGF-I receptor; IR; insulin receptor; SFM; serum-free medium; PI; 3-ki; phosphatidylinositol; 3; kinase; IRS; insulin receptor substrate; MEF; mouse embryo fibroblasts; Y; tyrosine residue;
D O I
10.1055/s-2007-978703
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
R-cells are mouse embryo fibroblasts with a targeted disruption of the insulin-like growth factor I receptor (ICF-IR) genes. Because R-cells do not express the IGF-IR, they are ideal for studying the biological effects of the insulin receptor (IR), independently from any contribution by the IGF-IR. By stably transfecting R-cells with constructs expressing the IR, we show here the IR can protect cells from apoptosis induced by anoikis or by okadaic acid. The IR, however, is not as efficient as the IGF-IR in protecting mouse embryo Fibroblasts from apoptosis, even when IRS-l, one of its major substrates, is over-expressed. In addition, the protection by the IGF-IR is resistant to inhibitors of phosphatidylinositol 3-kinase (PI 3-ki), while the anti-apoptotic effect of the IR is sensitive. These experiments suggest that the IGF-IR uses an alternative anti-apoptotic pathway, not shared with the IR, which is PI3-ki-independent.
引用
收藏
页码:80 / 89
页数:10
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