A transcriptomic approach to predict the impact of β-(1,3)-polyglucuronic acid sodium salt and derivatives in the main biological processes

被引：4

作者：

Delattre, C. ^{[1
]}

Michaud, P. ^{[2
]}

Chaisemartin, L. ^{[1
]}

Berthon, J. Y. ^{[1
]}

Rios, L. ^{[1
,3
]}

机构：

[1] GREENTECH Biotechnol, F-63360 St Beauzire, France

[2] Univ Blaise Pascal Polytech Clermont Ferrand, Lab Genie Chim & Biochim, F-63174 Aubiere, France

[3] ALTINAT, F-63360 St Beauzire, France

来源：

CARBOHYDRATE POLYMERS | 2012年 / 87卷 / 02期

关键词：

beta-(1,3)-Polyglucuronic acid sodium salt; Biological processes; Curdlan; DNA-array; Regioselective oxidation; Transcriptomic analysis; VITRO ANTIOXIDANT ACTIVITY; MOLECULAR-WEIGHT; ANTICOAGULANT; OLIGOSACCHARIDES; POLYSACCHARIDE; PROTEIN-4; FUCOIDAN; CURDLAN;

D O I：

10.1016/j.carbpol.2011.10.010

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

beta-(1,3)-Polyglucuronic acid sodium salt was produced by the regioselective oxidation of curdlan using 2,2,6,6,-tetramethylpiperidine-1-oxyl radical (TEMPO)/NaBr/NaClO systems. This beta-(1,3)-polyuronic acid sodium salt was depolymerized, O-sulphated and O-acetylated. All these molecules have been biologically screened for transcriptomic analysis using DNA array. The transcriptomical responses were studied after statistical gene ontology in order to evaluate biological responses disturbed by the individual treatments of human fibroblast by all these beta-(1,3)-polyglucuronic acid sodium salt derivatives. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1828 / 1836

页数：9

共 35 条

[1] DNA microarray and genes expression patterns Part one: design and use [J].