Criticality of the biological and physical stimuli array inducing resident cardiac stem cell determination

被引:79
作者
Forte, Giancarlo [1 ,2 ]
Carotenuto, Felicia [1 ]
Pagliari, Francesca [1 ]
Pagliari, Stefania
Cossa, Paolo [1 ]
Fiaccavento, Roberta [1 ]
Ahiuwalia, Arti [3 ]
Vozzi, Giovanni [3 ]
Vinci, Bruna [3 ]
Serafino, Annalucia [4 ]
Rinaldi, Antonio [5 ,6 ]
Traversa, Enrico [5 ,6 ]
Carosella, Luciana [7 ]
Minieri, Marilena [1 ,6 ]
Di Nardo, Paolo [1 ,6 ]
机构
[1] Univ Roma Tor Vergata, Dipartimento Med Interna, Lab Cardiol Mol & Cellulare, Via Montpellier 1, I-00133 Rome, Italy
[2] INRC, Bologna, Italy
[3] Univ Pisa, Ctr Interdipartimentale Ric E Piaggio, Pisa, Italy
[4] CNR, Ist Neurobiol & Med Mol, Rome, Italy
[5] Univ Roma Tor Vergata, Dipartimento Sci Tecnol Chim, I-00133 Rome, Italy
[6] Univ Roma Tor Vergata, Ctr Nanosci Nanotecnol & Strumentaz Innovat, I-00133 Rome, Italy
[7] Univ Cattolica Sacro Cuore, Ist Med Interna & Geriatria, Rome, Italy
关键词
mesenchymal stem cells; cardiac progenitor cells; three-dimensional scaffolds; tissue engineering;
D O I
10.1634/stemcells.2008-0061
中图分类号
Q813 [细胞工程];
学科分类号
摘要
The replacement of injured cardiac contractile cells with stem cell-derived functionally efficient cardiomyocytes has been envisaged as the resolutive treatment for degenerative heart diseases. Nevertheless, many technical issues concerning the optimal procedures to differentiate and engraft stem cells remain to be answered before heart cell therapy could tie routinely used in clinical practice. So far, most studies have been focused on evaluating the differentiative potential of different growth factors without considering that only the synergistic cooperation of biochemical, topographic, chemical, and physical factors could induce stem cells to adopt the desired phenotype. The present study demonstrates that the differentiation of cardiac progenitor cells to cardiomyocytes does not occur when cells are challenged with soluble growth factors alone, but requires strictly controlled procedures for the isolation of a progenitor cell population and the artifactual recreation of a microenvironment critically featured by a fine-tuned combination of specific biological and physical factors. Indeed, the scaffold geometry and stiffness are crucial in enhancing growth factor differentiative effects on progenitor cells. The exploitation of this concept could be essential in setting up suitable procedures to fabricate functionally efficient engineered tissues.
引用
收藏
页码:2093 / 2103
页数:11
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