Indirect immunofluorescence on HEp-2000 substrate is a sensitive method for detecting anti-Ro/SSA antibodies in patients with lupus erythematosus and/or photosensitivity

被引：0

作者：

Cozzani, Emanuele ^{[1
]}

Drosera, Massimo ^{[1
]}

Siccardi, Marina ^{[1
]}

Babbini, Giovanni ^{[1
]}

Parodi, Aurora ^{[1
]}

机构：

[1] Technogenet Bouty Grp, Milan, Italy

来源：

JOURNAL OF RHEUMATOLOGY | 2008年 / 35卷 / 07期

关键词：

HEp-2000; cells; anti-Ro/SSA antibodies; high sensitivity; lupus erythematosus;

D O I：

暂无

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective. To evaluate the sensitivity of indirect immunofluorescence (IIF) utilizing HEp-2000 cells to detect antinuclear and anti-Ro/SSA antibodies. Methods. We examined 5949 sera samples from 2316 patients with connective tissue diseases. All sera were tested by IIF on HEp-2 cells as substrate and by counterimmunoelectrophoresis (CIE). The positive sera for anti-Ro/SSA antibodies by CIE were retested by IIF using HEp-2000 cells. The sera that were anti-Ro/SSA-positive in CIE but negative on HEp-2000 cells were tested by ELISA for the 60 and 52 subunits of Ro/SSA antigen. Results. Our results confirm the high sensitivity (81%) of HEp-2000 cells in detecting anti Ro/SSA antibodies. Conclusion. Using the HEp-2000 substrate is a reliable, simple alternative method for detecting anti-Ro/SSA antibodies in a first-level screening test such as IIF, especially in patients with lupus erythematosus and/or photosensitivity.

引用

页码：1320 / 1322

页数：3

共 15 条

[1] STATISTICS NOTES - DIAGNOSTIC-TESTS-1 - SENSITIVITY AND SPECIFICITY .3. [J].