Monitoring of organophosphorus pesticides in vegetables using monoclonal antibody-based direct competitive ELISA followed by HPLC-MS/MS

被引：70
作者：
Xu, Zhen-Lin [1 ]
Deng, Hao [1 ]
Deng, Xing-Fei [1 ]
Yang, Jin-Yi [1 ]
Jiang, Yue-Ming [2 ]
Zeng, Dao-Ping [1 ]
Huang, Fang [3 ]
Shen, Yu-Dong [1 ]
Lei, Hong-Tao [1 ]
Wang, Hong [1 ]
Sun, Yuan-Ming [1 ]
机构：
[1] S China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Guangdong, Peoples R China
[2] Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China
[3] China Natl Analyt Ctr, Guangzhou 510070, Guangdong, Peoples R China
来源：
FOOD CHEMISTRY | 2012年 / 131卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Direct competitive ELISA; Organophosphorus pesticides; Matrix effect; Solid-phase extraction; HPLC-MS/MS; ACCELERATED SOLVENT-EXTRACTION; BROAD-SPECIFICITY IMMUNOASSAY; LINKED-IMMUNOSORBENT-ASSAY; PYRETHROID INSECTICIDES; ENVIRONMENTAL-SAMPLES; ENZYME-IMMUNOASSAY; RESIDUES; HERBICIDES; FOOD; COMPONENTS;
D O I：
10.1016/j.foodchem.2011.10.020
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A multi-analytes method for monitoring of organophosphorus pesticides (UPS) using a combination of broad-specificity direct competitive enzyme-linked immunosorbent assay (dcELISA) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. The reaction formats of dcELISA as well as the matrix effects of vegetable samples by different treatments were studied. The dcELSIA based on horseradish peroxidase-labelled monoclonal antibody and solid-phase extraction can analyse 42 samples in duplicate simultaneously for 12 OPs with a limit of detection at 20 mu g L(-1) within 40 min, with good accuracy and reproducibility. For screening purpose, the dcELISA can distinguish positive samples from hundreds of negative samples at a rapid, high-throughput and low cost manner. The positive samples can be following confirmed by HPLC-MS/MS for the kinds and the relative amounts of OPs. The method is suitable for monitoring of OP contamination in vegetables samples with high-efficiency and low cost. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1569 / 1576
页数：8
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