Monitoring of organophosphorus pesticides in vegetables using monoclonal antibody-based direct competitive ELISA followed by HPLC-MS/MS

被引：70

作者：

Xu, Zhen-Lin ^{[1
]}

Deng, Hao ^{[1
]}

Deng, Xing-Fei ^{[1
]}

Yang, Jin-Yi ^{[1
]}

Jiang, Yue-Ming ^{[2
]}

Zeng, Dao-Ping ^{[1
]}

Huang, Fang ^{[3
]}

Shen, Yu-Dong ^{[1
]}

Lei, Hong-Tao ^{[1
]}

Wang, Hong ^{[1
]}

Sun, Yuan-Ming ^{[1
]}

机构：

[1] S China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Guangdong, Peoples R China

[2] Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China

[3] China Natl Analyt Ctr, Guangzhou 510070, Guangdong, Peoples R China

来源：

FOOD CHEMISTRY | 2012年 / 131卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Direct competitive ELISA; Organophosphorus pesticides; Matrix effect; Solid-phase extraction; HPLC-MS/MS; ACCELERATED SOLVENT-EXTRACTION; BROAD-SPECIFICITY IMMUNOASSAY; LINKED-IMMUNOSORBENT-ASSAY; PYRETHROID INSECTICIDES; ENVIRONMENTAL-SAMPLES; ENZYME-IMMUNOASSAY; RESIDUES; HERBICIDES; FOOD; COMPONENTS;

D O I：

10.1016/j.foodchem.2011.10.020

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A multi-analytes method for monitoring of organophosphorus pesticides (UPS) using a combination of broad-specificity direct competitive enzyme-linked immunosorbent assay (dcELISA) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. The reaction formats of dcELISA as well as the matrix effects of vegetable samples by different treatments were studied. The dcELSIA based on horseradish peroxidase-labelled monoclonal antibody and solid-phase extraction can analyse 42 samples in duplicate simultaneously for 12 OPs with a limit of detection at 20 mu g L(-1) within 40 min, with good accuracy and reproducibility. For screening purpose, the dcELISA can distinguish positive samples from hundreds of negative samples at a rapid, high-throughput and low cost manner. The positive samples can be following confirmed by HPLC-MS/MS for the kinds and the relative amounts of OPs. The method is suitable for monitoring of OP contamination in vegetables samples with high-efficiency and low cost. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1569 / 1576

页数：8

共 25 条

[11] Determination of pesticides by enzyme immunoassay [J].

Morozova, VS ;

Levashova, AI ;

Eremin, SA .

JOURNAL OF ANALYTICAL CHEMISTRY, 2005, 60 (03) :202-217

[12] Analysis of pesticides in food and environmental samples by enzyme-linked immunosorbent assays [J].

Nunes, GS ;

Toscano, IA ;

Barcelo, D .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1998, 17 (02) :79-87

[13] Determination of organophosphorus pesticides in foods using an accelerated solvent extraction system [J].

Obana, H ;

Kikuchi, K ;

Okihashi, M ;

Hori, S .

ANALYST, 1997, 122 (03) :217-220

[14] Development of ELISAs for the Class-Specific Determination of Organophosphorus Pesticides [J].

Piao, Yuan Zhe ;

Kim, Yoo Jung ;

Kim, Young Ah ;

Lee, Hye-Sung ;

Hammock, Bruce D. ;

Lee, Yong Tae .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (21) :10004-10013

[15] Analytical methods for estimation of organophosphorus pesticide residues in fruits and vegetables: A review [J].

Sharma, Dipakshi ;

Nagpal, Avinash ;

Pakade, Yogesh B. ;

Katnoria, Jatinder Kaur .

TALANTA, 2010, 82 (04) :1077-1089

[16] Evaluation of a solid-phase extraction dual-layer carbon/primary secondary amine for clean-up of fatty acid matrix components from food extracts in multiresidue pesticide analysis [J].

Shimelis, Olga ;

Yang, Yuhui ;

Stenerson, Katherine ;

Kaneko, Toshiro ;

Ye, Michael .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1165 (1-2) :18-25

[17] Broad-specificity immunoassay of low molecular weight food contaminants: new paths to Utopia! [J].

Spinks, CA .

TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2000, 11 (06) :210-217

[18] Immunoanalytical techniques for analyzing pesticides in the environment [J].

Suri, C. Raman ;

Boro, Robin ;

Nangia, Yogesh ;

Gandhi, Sonu ;

Sharma, Priyanka ;

Wangoo, Nishima ;

Rajesh, Kumar ;

Shekhawat, G. S. .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2009, 28 (01) :29-39

[19] OPTIMIZATION OF THE COVALENT CONJUGATING PROCEDURE (NAIO4) OF HORSERADISH-PEROXIDASE TO ANTIBODIES FOR USE IN ENZYME-LINKED-IMMUNOSORBENT-ASSAY [J].

TSANG, VCW ;

GREENE, RM ;

PILCHER, JB .

JOURNAL OF IMMUNOASSAY, 1995, 16 (04) :395-418

[20] Development of a class-specific immunoassay for the type I pyrethroid insecticides [J].

Watanabe, T ;

Shan, G ;

Stoutamire, DW ;

Gee, SJ ;

Hammock, BD .

ANALYTICA CHIMICA ACTA, 2001, 444 (01) :119-129

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