Analytical evaluation of the ADAMS A1c HA8180T analyzer for the measurement of HbA1c

被引:8
作者
Urrechaga, Eloisa [1 ]
机构
[1] Hosp Galdakao Usansolo, Galdakao, Vizcaya, Spain
关键词
diabetes; HA-8180T analyzer; HbA1c; HPLC;
D O I
10.1002/jcla.22155
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
BackgroundThe ADAMS A1c HA-8180T (ARKRAY, Inc., Kyoto, Japan) is a high pressure liquid chromatography system designed for separation and quantitation of HbA1c, while detecting hemoglobin (Hb) variants and HbA(2), with an analysis time of just 3.5min. The aim was to evaluate the analytical performance of this analyzer for routine HbA1c quantitation. MethodsTrueness, imprecision, carry over, linearity, the effect of anemia and correlation with ADAMS A1c HA-8180V (in Variant mode) were assessed according to guidelines of the Clinical and Laboratory Standards Institute (CLSI) and manufacturer. The presence of coexisting interfering chemically modified Hb variants was also assessed. ResultsThe results were found to be accurate (total error of 1.74%) and precise: within-run, between-run, between-day and total coefficients of variation were 0.71%, 0.77%, 0.36%, and 0.93% at low concentrations, and 0.43%, 0.29%, 0%, and 0.48% at high concentrations of HbA1c. Carry over, linearity, and correlation of methods were excellent. HbA1c measurement was independent of total Hb concentration in a range 40-214g/L, and shows no interference from Hb chemically modified commonly observed in this type of analysis, while common Hb variants are detected. ConclusionThe analyzer is a suitable system for glycemic control in patients with diabetes and the detection of Hb variants.
引用
收藏
页数:6
相关论文
共 14 条
  • [1] [Anonymous], RES J CHEM SCI
  • [2] Using the likelihood ratio to evaluate allowable total error - an example with glycated hemoglobin (HbA1c)
    Asberg, Arne
    Odsaeter, Ingrid Hov
    Carlsen, Sven Magnus
    Mikkelsen, Gustav
    [J]. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2015, 53 (09) : 1459 - 1464
  • [3] Conditions associated with very low values of glycohaemoglobin measured by an HPLC method
    Camargo, JL
    Gross, JL
    [J]. JOURNAL OF CLINICAL PATHOLOGY, 2004, 57 (04) : 346 - 349
  • [4] Haemoglobin A1c: Historical overview and current concepts
    Lenters-Westra, Erna
    Schindhelm, Roger K.
    Bilo, Henk J.
    Slingerland, Robbert J.
    [J]. DIABETES RESEARCH AND CLINICAL PRACTICE, 2013, 99 (02) : 75 - 84
  • [5] Haemoglobin A1c measurement in patients with chronic kidney disease
    Li, Qing
    Ju, Yi
    Jin, Ting
    Pang, Biqiong
    Deng, Jiade
    Du, Tongxin
    Wang, Hong
    [J]. CLINICAL BIOCHEMISTRY, 2014, 47 (06) : 481 - 484
  • [6] Little Randie R, 2015, J Diabetes Sci Technol, V9, P849, DOI 10.1177/1932296815572367
  • [7] Measurement of Hba1c in patients with chronic renal failure
    Little, Randie R.
    Rohlfing, Curt L.
    Tennill, Alethea L.
    Hanson, Steven E.
    Connolly, Shawn
    Higgins, Trefor
    Wiedmeyer, Charles E.
    Weykamp, Cas W.
    Krause, Richard
    Roberts, William
    [J]. CLINICA CHIMICA ACTA, 2013, 418 : 73 - 76
  • [8] International Expert Committee Report on the Role of the A1C Assay in the Diagnosis of Diabetes
    Nathan, David M.
    Nathan, David M.
    Balkau, Beverly
    Bonora, Enzo
    Borch-Johnsen, Knut
    Buse, John B.
    Colagiuri, Stephen
    Davidson, Mayer B.
    DeFronzo, Ralph
    Genuth, Saul
    Holman, Rury R.
    Ji, Linong
    Kirkman, Sue
    Knowler, William C.
    Schatz, Desmond
    Shaw, Jonathan
    Sobngwi, Eugene
    Steffes, Michael
    Vaccaro, Olga
    Wareham, Nick
    Zinman, Bernard
    Kahn, Richard
    [J]. DIABETES CARE, 2009, 32 (07) : 1327 - 1334
  • [9] NATHAN DM, 1982, CLIN CHEM, V28, P512
  • [10] THE EFFECT OF LONG-TERM INTENSIFIED INSULIN-TREATMENT ON THE DEVELOPMENT OF MICROVASCULAR COMPLICATIONS OF DIABETES-MELLITUS
    REICHARD, P
    NILSSON, BY
    ROSENQVIST, U
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1993, 329 (05) : 304 - 309