Hydrolytic Enzyme of Cellulose for Complex Formulation Applied Research

被引：19

作者：

Lin, Zeng-Xiang ^{[1
]}

Zhang, Hong-Man ^{[2
]}

Ji, Xiao-Jun ^{[1
]}

Chen, Jing-Wen ^{[1
]}

Huang, He ^{[1
]}

机构：

[1] Nanjing Univ Technol, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Peoples R China

[2] Nanjing Univ Technol, Dept Appl Chem, Nanjing 210009, Peoples R China

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2011年 / 164卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Experimental mixture design; Cellulose; Complex formulation; Cellulase; Xylanase; CORN STOVER; OPTIMIZATION; DESIGN; LIGNOCELLULOSE; ENDOGLUCANASE; PERFORMANCE; CELLULASES; SYNERGISM; BIOFUELS; XYLANASE;

D O I：

10.1007/s12010-010-9111-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To improve the enzymatic hydrolytic efficiency and reduce the supplementation of enzymes, the mixture designed experimental approach was used to optimize the composition of enzyme mixture and promote the hydrolysis of ball-milled corn stover. From the experimental results, a synergistic effect was found when combinations of the three enzymes, two kinds of cellulases and a kind of xylanase, were used. The optimal hydrolysis of pretreated corn stover accorded with enzymes activity ration of FPU/CMCase/beta-glucosidase/xylanase = 4.4:1:75:829, and the hydrolysis efficiency of corn stover increased significantly compared with using individual enzyme. The results indicated that the mixture design experiment could be an effective tool for optimized enzyme mixture for lignocellulose hydrolysis.

引用

页码：23 / 33

页数：11

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