Perfusion System for Cell-Scaffold Complex Culture in Vitro

被引：0

作者：

Zhang, Chunqiu ^{[1
]}

Wu, Pengfei ^{[1
]}

Wang, Xin ^{[1
]}

Gao, Lilan ^{[1
]}

机构：

[1] Tianjin Univ Technol, Tianjin Key Lab Adv Mechatron Syst Design & Intel, Expt Mech & Elect Engn Educ, Tianjin 300384, Peoples R China

来源：

2019 IEEE INTERNATIONAL CONFERENCE ON MECHATRONICS AND AUTOMATION (ICMA) | 2019年

基金：

中国国家自然科学基金;

关键词：

cell; perfusion system; Fluid shear;

D O I：

10.1109/icma.2019.8816526

中图分类号：

TP [自动化技术、计算机技术];

学科分类号：

0812 ;

摘要：

The biological scaffold can be applied to the stereo culture of cells to provide a good living environment. Stimulation of cells in bioreactors is a key area of research in biomechanics and regenerative medicine; it can create a three-dimensional space similar to the in vivo environment to proliferate high-quality cells that are difficult to culture in culture systems. The organic combination of cell tissue engineering and in vitro perfusion culture is the key technology for in vitro culture of cell tissue. A new type of in vitro perfusion system has been developed in this paper, basing on laboratory universal consumables and six-well plates, combining different bracket material shapes, including cuboids, cubes, cylinders, and triangular prisms, Designing a reasonable mold with reverse thinking, Sealing caps suitable for four different scaffolds were prepared by 3D printing and pouring technology. With the use of peristaltic pumps, catheters, liquid storage bottle, joints, etc., an in vitro perfusion system for cell tissue engineering is built. The system has the characteristics of simple operation, flexible collocation and strong practicability.

引用

页码：475 / 479

页数：5