Odd-chain polyunsaturated fatty acids in thraustochytrids

被引：38

作者：

Chang, Kim Jye Lee ^{[1
,3
]}

Mansour, Maged P. ^{[2
]}

Dunstan, Graeme A. ^{[1
]}

Blackburn, Susan I. ^{[1
]}

Koutoulis, Anthony ^{[3
]}

Nichols, Peter D. ^{[2
]}

机构：

[1] CSIRO Marine & Atmospher Res, Energy Transformed Natl Res Flagship, Hobart, Tas 7001, Australia

[2] CSIRO Marine & Atmospher Res, Food Futures Natl Res Flagship, Hobart, Tas 7001, Australia

[3] Univ Tasmania, Sch Plant Sci, Hobart, Tas 7001, Australia

来源：

PHYTOCHEMISTRY | 2011年 / 72卷 / 11-12期

关键词：

Thraustochytrids; Odd-carbon fatty acids; Polyenoic fatty acids; ALGAE-LIKE MICROORGANISMS; MASS-SPECTROMETRY; HETEROTROPHIC PRODUCTION; MERCENARIA-MERCENARIA; PONTOPOREIA-FEMORATA; MARINE PROTISTS; IDENTIFICATION; FUNGI; PARASITE; OPTIMIZATION;

D O I：

10.1016/j.phytochem.2011.04.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of unusual odd-chain fatty acids (OC-FA) were identified in two thraustochytrid strains. TC 01 and TC 04, isolated from waters off the south east coast of Tasmania, Australia. FA compositions were determined by capillary GC and GC-MS, with confirmation of polyunsaturated fatty acids (PUFA) structure performed by analysis of 4,4-dimethyloxazoline derivatives. PUFA constituted 68-74% of the total FA, with the essential PUFA; eicosapentaenoic acid (20:5(0, EPA), arachidonic acid (20:40)6, AA) and docosahexaenoic acid (22:6 omega 3. DHA), accounting for 42-44% of the total FA. High proportions of the saturated OC-FA 15:0(7.1% in TC 01) and 17:0(6.2% in TC 04) were detected. The OC-FA 17:1 omega 8 was present at 2.8% in TC 01. Of particular interest, the C(21) PUFA 21:5 omega 5 and 21:4 omega 7 were detected at 3.5% and 4.1%, respectively, in TC 04. A proposed biosynthesis pathway for these OC-PUFA is presented. It is possible that the unsaturated OC-PUFA found previously in a number of marine animals were derived from dietary thraustochytrids and they could be useful biomarkers in environmental and food web studies. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1460 / 1465

页数：6

共 39 条

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