Tissue microarrays in drug discovery

被引:152
作者
Sauter, G
Simon, R
Hillan, K
机构
[1] Univ Basel, Inst Pathol, CH-4031 Basel, Switzerland
[2] Genentech Inc, San Francisco, CA 94080 USA
关键词
D O I
10.1038/nrd1254
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Advances in molecular methods have massively facilitated the discovery of potential molecular targets for gene-specific therapy. Accelerated lead discovery has at the same time generated a massive demand for thorough validation of such putative targets. Very often human tissue analysis is needed for this purpose. However, the need to analyse large numbers of well-characterized human tissues constitutes a major bottleneck in drug discovery and development. Traditional tissue analysis in a slide-by-slide manner is slow, expensive and difficult to standardize. In addition, precious specimens, such as tissue samples from clinical studies, are usually exhausted after a few analyses. The tissue microarray technology overcomes these shortcomings as it allows the simultaneous analysis of up to 1,000 minute tissue samples in a single experiment. This article will review how high-throughput tissue microarray analyses can dramatically facilitate translational research at several different levels.
引用
收藏
页码:962 / 972
页数:11
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