Markers of epithelial-mesenchymal transition and epithelial differentiation in sarcomatoid carcinoma: Utility in the differential diagnosis with sarcoma

被引:36
作者
Cates, Justin M. M. [1 ,2 ]
Dupont, William D. [3 ,4 ]
Barnes, Jason W. [1 ]
Edmunds, Hayward S. [5 ,6 ]
Fasig, John H. [1 ]
Olson, Sandra J. [1 ]
Black, Candice C. [5 ,6 ]
机构
[1] Vanderbilt Univ, Med Ctr, Dept Pathol, Nashville, TN 37232 USA
[2] Vanderbilt Univ, Med Ctr, Ctr Bone Biol, Nashville, TN 37232 USA
[3] Vanderbilt Univ, Med Ctr, Dept Biostat, Nashville, TN 37232 USA
[4] Vanderbilt Univ, Sch Med, Nashville, TN 37232 USA
[5] Dartmouth Hitchcock Med Ctr, Dept Pathol, Lebanon, NH 03766 USA
[6] Dartmouth Med Sch, Lebanon, NH USA
来源
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY | 2008年 / 16卷 / 03期
关键词
sarcomatoid carcinoma; spindle cell carcinoma; carcinosarcoma; sarcoma; epithelial-inesenchyrnal transition;
D O I
10.1097/PAI.0b013e318156e9b4
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The distinction between sarcomatoid carcinoma (SC) and bona fide sarcoma can be difficult using conventional immunohistochemical markers. Epithelial-inesenchymal transition (EMT) has been proposed as a histogenetic mechanism for the development of SC. Expression of selected markers of EMT (Twist and Slug) was compared with other markers of epithelial differentiation in SC and spindle cell sarcoma to determine the utility of these antigens in this differential diagnosis. Twenty-seven cases of SC (excluding those of gynecologic origin) were stained by immunohistochemistry for cytokeratins (AE1/AE3, 5D3, CK5/6, and 34 beta E12), p63, claudin-1, claudin-7, epithelial cadherin, placental cadherin, epithelial cell adhesion molecule/ epithelial-specific antigen, 14-3-3 sigma, Twist, and Slug. A comparison group of 21 spindle or pleomorphic spindle cell sarcomas was also studied. Immunohistochemical stains were scored in a semiquantitative manner and subsequent exploratory analyses were performed using logistic regression and chi(2) tests. Only cytokeratin AE1/AE3 specifically labeled SC in a statistically significant manner. Other epithelia I-specific markers tested did not distinguish SC from sarcoma primarily owing to low sensitivity. However, when positive, immunostains Such as CK5/6, membranous epithelial cadherin, and unclear p63 may aid in the distinction of SC from sarcoma. EMT markers were expressed in most cases of both SC and sarcoma, and were not useful in making a differential diagnosis between these neoplasms.
引用
收藏
页码:251 / 262
页数:12
相关论文
共 70 条
[1]   Anti-mesothelial markers in sarcomatoid mesothelioma and other spindle cell neoplasms [J].
Attanoos, RL ;
Dojcinov, SD ;
Webb, R ;
Gibbs, AR .
HISTOPATHOLOGY, 2000, 37 (03) :224-231
[2]   Importance of P-cadherin, β-catenin, and Wnt5a/frizzled for progression of melanocytic tumors and prognosis in cutaneous melanoma [J].
Bachmann, IM ;
Straume, O ;
Puntervoll, HE ;
Kalvenes, MB ;
Akslen, LA .
CLINICAL CANCER RESEARCH, 2005, 11 (24) :8606-8614
[3]   SARCOMATOID CARCINOMA - AN ULTRASTRUCTURAL-STUDY WITH LIGHT-MICROSCOPIC AND IMMUNOHISTOCHEMICAL CORRELATION OF 10 CASES FROM VARIOUS ANATOMIC SITES [J].
BALERCIA, G ;
BHAN, AK ;
DICKERSIN, GR .
ULTRASTRUCTURAL PATHOLOGY, 1995, 19 (04) :249-263
[4]   The Snail genes as inducers of cell movement and survival: implications in development and cancer [J].
Barrallo-Gimeno, A ;
Nieto, MA .
DEVELOPMENT, 2005, 132 (14) :3151-3161
[5]   Sarcomatoid carcinomas of the upper aerodigestive tracts [J].
Batsakis, JG ;
Suarez, P .
ADVANCES IN ANATOMIC PATHOLOGY, 2000, 7 (05) :282-293
[6]   Correlation of Snail expression with histological grade and lymph node status in breast carcinomas [J].
Blanco, MJ ;
Moreno-Bueno, G ;
Sarrio, D ;
Locascio, A ;
Cano, A ;
Palacios, J ;
Nieto, MA .
ONCOGENE, 2002, 21 (20) :3241-3246
[7]   The transcription factor Slug represses E-cadherin expression and induces epithelial to mesenchymal transitions:: a comparison with Snail and E47 repressors [J].
Bolós, V ;
Peinado, H ;
Pérez-Moreno, MA ;
Fraga, MF ;
Esteller, M ;
Cano, A .
JOURNAL OF CELL SCIENCE, 2003, 116 (03) :499-511
[8]  
BRENNAN MF, 2005, CANC PRINCIPLES PRAC, P1582
[9]   Inducible expression of Snail selectively increases paracellular ion permeability and differentially modulates tight junction proteins [J].
Carrozzino, F ;
Soulié, P ;
Huber, D ;
Mensi, N ;
Orci, L ;
Cano, A ;
Féraille, E ;
Montesano, R .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2005, 289 (04) :C1002-C1014
[10]   Claudin-1 regulates cellular transformation and metastatic behavior in colon cancer [J].
Dhawan, P ;
Singh, AB ;
Deane, NG ;
No, Y ;
Shiou, SR ;
Schmidt, C ;
Neff, J ;
Washington, MK ;
Beauchamp, RD .
JOURNAL OF CLINICAL INVESTIGATION, 2005, 115 (07) :1765-1776