Targeted resequencing of a genomic region influencing tameness and aggression reveals multiple signals of positive selection

被引：22

作者：

Albert, F. W. ^{[1
]}

Hodges, E. ^{[2
]}

Jensen, J. D. ^{[3
]}

Besnier, F. ^{[4
]}

Xuan, Z. ^{[2
]}

Rooks, M. ^{[2
]}

Bhattacharjee, A. ^{[5
]}

Brizuela, L. ^{[5
]}

Good, J. M. ^{[1
]}

Green, R. E. ^{[1
]}

Burbano, H. A. ^{[1
]}

Plyusnina, I. Z. ^{[6
]}

Trut, L. ^{[6
]}

Andersson, L. ^{[7
]}

Schoeneberg, T. ^{[8
]}

Carlborg, O. ^{[4
]}

Hannon, G. J. ^{[2
]}

Paabo, S. ^{[1
,9
]}

机构：

[1] Max Planck Inst Evolutionary Anthropol, Dept Evolutionary Genet, D-04103 Leipzig, Germany

[2] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

[3] Univ Massachusetts, Sch Med, Program Bioinformat & Integrat Biol, Worcester, MA USA

[4] Swedish Univ Agr Sci, Dept Anim Breeding & Genet, S-75007 Uppsala, Sweden

[5] Agilent Technol, Life Sci Grp, Santa Clara, CA USA

[6] Russian Acad Sci, Inst Cytol & Genet, Siberian Branch, Novosibirsk 630090, Russia

[7] Uppsala Univ, Dept Med Biochem & Microbiol, Uppsala, Sweden

[8] Univ Leipzig, Inst Biochem, Leipzig, Germany

[9] Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol, Uppsala, Sweden

来源：

HEREDITY | 2011年 / 107卷 / 03期

基金：

瑞典研究理事会;

关键词：

high-throughput sequencing; sequence capture; positive selection; behavior; QTL mapping; MOLECULAR POPULATION-GENETICS; REDUCED AGGRESSIVENESS; SOFT SWEEPS; HITCHHIKING; ADAPTATION; BEHAVIOR; GENES; SIGNATURE; MUTATION; MODEL;

D O I：

10.1038/hdy.2011.4

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

The identification of the causative genetic variants in quantitative trait loci (QTL) influencing phenotypic traits is challenging, especially in crosses between outbred strains. We have previously identified several QTL influencing tameness and aggression in a cross between two lines of wild-derived, outbred rats (Rattus norvegicus) selected for their behavior towards humans. Here, we use targeted sequence capture and massively parallel sequencing of all genes in the strongest QTL in the founder animals of the cross. We identify many novel sequence variants, several of which are potentially functionally relevant. The QTL contains several regions where either the tame or the aggressive founders contain no sequence variation, and two regions where alternative haplotypes are fixed between the founders. A re-analysis of the QTL signal showed that the causative site is likely to be fixed among the tame founder animals, but that several causative alleles may segregate among the aggressive founder animals. Using a formal test for the detection of positive selection, we find 10 putative positively selected regions, some of which are close to genes known to influence behavior. Together, these results show that the QTL is probably not caused by a single selected site, but may instead represent the joint effects of several sites that were targets of polygenic selection. Heredity (2011) 107, 205-214; doi: 10.1038/hdy.2011.4;published online 9 February 2011

引用

页码：205 / 214

页数：10

共 48 条

[1] Phenotypic differences in behavior, physiology and neurochemistry between rats selected for tameness and for defensive aggression towards humans [J].

Albert, Frank W. ;

Shchepina, Olesya ;

Winter, Christine ;

Roempler, Holger ;

Teupser, Daniel ;

Palme, Rupert ;

Ceglarek, Uta ;

Kratzsch, Juergen ;

Sohr, Reinhard ;

Trut, Lyudmila N. ;

Thiery, Joachim ;

Morgenstern, Rudolf ;

Plyusnina, Irina Z. ;

Schoeneberg, Torsten ;

Paabo, Svante .

HORMONES AND BEHAVIOR, 2008, 53 (03) :413-421

[2] Genetic Architecture of Tameness in a Rat Model of Animal Domestication [J].

Albert, Frank W. ;

Carlborg, Orjan ;

Plyusnina, Irina ;

Besnier, Francois ;

Hedwig, Daniela ;

Lautenschlaeger, Susann ;

Lorenz, Doreen ;

McIntosh, Jenny ;

Neumann, Christof ;

Richter, Henning ;

Zeising, Claudia ;

Kozhemyakina, Rimma ;

Shchepina, Olesya ;

Kratzsch, Juergen ;

Trut, Lyudmila ;

Teupser, Daniel ;

Thiery, Joachim ;

Schoeneberg, Torsten ;

Andersson, Leif ;

Paabo, Svante .

GENETICS, 2009, 182 (02) :541-554

[3] A map of human genome variation from population-scale sequencing [J].

Altshuler, David ;

Durbin, Richard M. ;

Abecasis, Goncalo R. ;

Bentley, David R. ;

Chakravarti, Aravinda ;

Clark, Andrew G. ;

Collins, Francis S. ;

De la Vega, Francisco M. ;

Donnelly, Peter ;

Egholm, Michael ;

Flicek, Paul ;

Gabriel, Stacey B. ;

Gibbs, Richard A. ;

Knoppers, Bartha M. ;

Lander, Eric S. ;

Lehrach, Hans ;

Mardis, Elaine R. ;

McVean, Gil A. ;

Nickerson, DebbieA. ;

Peltonen, Leena ;

Schafer, Alan J. ;

Sherry, Stephen T. ;

Wang, Jun ;

Wilson, Richard K. ;

Gibbs, Richard A. ;

Deiros, David ;

Metzker, Mike ;

Muzny, Donna ;

Reid, Jeff ;

Wheeler, David ;

Wang, Jun ;

Li, Jingxiang ;

Jian, Min ;

Li, Guoqing ;

Li, Ruiqiang ;

Liang, Huiqing ;

Tian, Geng ;

Wang, Bo ;

Wang, Jian ;

Wang, Wei ;

Yang, Huanming ;

Zhang, Xiuqing ;

Zheng, Huisong ;

Lander, Eric S. ;

Altshuler, David L. ;

Ambrogio, Lauren ;

Bloom, Toby ;

Cibulskis, Kristian ;

Fennell, Tim J. ;

Gabriel, Stacey B. .

NATURE, 2010, 467 (7319) :1061-1073

[4]

[Anonymous], PLOS GENETICS

[5] Mice lacking PKCγ exhibit decreased anxiety [J].

Bowers, BJ ;

Collins, AC ;

Tritto, T ;

Wehner, JM .

BEHAVIOR GENETICS, 2000, 30 (02) :111-121

[6] Genome-wide analysis of a long-term evolution experiment with Drosophila [J].

Burke, Molly K. ;

Dunham, Joseph P. ;

Shahrestani, Parvin ;

Thornton, Kevin R. ;

Rose, Michael R. ;

Long, Anthony D. .

NATURE, 2010, 467 (7315) :587-U111

[7] AGGRESSIVE-BEHAVIOR AND ALTERED AMOUNTS OF BRAIN-SEROTONIN AND NOREPINEPHRINE IN MICE LACKING MAOA [J].

CASES, O ;

SEIF, I ;

GRIMSBY, J ;

GASPAR, P ;

CHEN, K ;

POURNIN, S ;

MULLER, U ;

AGUET, M ;

BABINET, C ;

SHIH, JC ;

DEMAEYER, E .

SCIENCE, 1995, 268 (5218) :1763-1766

[8] Fast and flexible simulation of DNA sequence data [J].

Chen, Gary K. ;

Marjoram, Paul ;

Wall, Jeffrey D. .

GENOME RESEARCH, 2009, 19 (01) :136-142

[9]

DARVASI A, 1995, GENETICS, V141, P1199

[10] Identification of the Bovine Arachnomelia Mutation by Massively Parallel Sequencing Implicates Sulfite Oxidase (SUOX) in Bone Development [J].

Droegemueller, Cord ;

Tetens, Jens ;

Sigurdsson, Snaevar ;

Gentile, Arcangelo ;

Testoni, Stefania ;

Lindblad-Toh, Kerstin ;

Leeb, Tosso .

PLOS GENETICS, 2010, 6 (08)

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