Continuous testing system for Baeyer-Villiger biooxidation using recombinant Escherichia coli expressing cyclohexanone monooxygenase encapsulated in polyelectrolyte complex capsules

被引:30
作者
Bucko, Marek [1 ]
Schenkmayerova, Andrea [1 ]
Gemeiner, Peter [1 ]
Vikartovska, Alica [1 ]
Mihovilovic, Marko D. [2 ]
Lacik, Igor [3 ]
机构
[1] Slovak Acad Sci, Dept Glycobiotechnol, Inst Chem, Ctr Glyc, SK-84538 Bratislava, Slovakia
[2] Vienna Univ Technol, Inst Appl Synthet Chem, A-1060 Vienna, Austria
[3] Slovak Acad Sci, Inst Polymer, SK-84541 Bratislava, Slovakia
关键词
Encapsulation; Polyelectrolyte complex capsules; Baeyer-Villiger biooxidation; Cyclohexanone monooxygenase; Flow calorimetry; Continuous packed bed reactor; CELLULOSE SULFATE-POLY(METHYLENE-CO-GUANIDINE) CAPSULES; ENANTIOMERICALLY PURE; ORGANIC-SYNTHESIS; BIOCATALYST; VERIFICATION; OXIDATIONS;
D O I
10.1016/j.enzmictec.2011.05.013
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An original strategy for universal laboratory testing of Baeyer-Villiger monooxygenases based on continuous packed-bed minireactor connected with flow calorimeter and integrated with bubble-free oxygenation is reported. Model enantioselective Baeyer-Villiger biooxidations of rac-bicyclo[3.2.0]hept-2-en-6-one to corresponding lactones (1R,5S)-3-oxabicyclo-[3.3.0]oct-6-en-3-one and (1S,5R)-2-oxabicyclo-[3.3.0]oct-6-en-3-one as important chiral synthons for the synthesis of bioactive compounds were performed in the minireactor equipped with a column packed with encapsulated recombinant cells Escherichia coli overexpressing cyclohexanone monooxygenase. The cells were encapsulated in polyelectrolyte complex capsules formed by reaction of oppositely charged polymers utilizing highly reproducible and controlled encapsulation process. Encapsulated cells tested in minireactor exhibited high operational stability with 4 complete substrate conversions to products and 6 conversions above 80% within 14 repeated consecutive biooxidation tests. Moreover, encapsulated cells showed high enzyme stability during 91 days of storage with substrate conversions above 80% up to 60 days of storage. Furthermore, usable thermometric signal of Baeyer-Villiger biooxidation obtained by flow calorimetry using encapsulated cells was utilized for preparatory kinetic study in order to guarantee sub-inhibitory initial substrate concentration for biooxidation tests. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:284 / 288
页数:5
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