Simultaneous Analysis of Multiple Cancer Biomarkers Using MALDI-TOF Mass Spectrometry Based on a Parylene-Matrix Chip

被引：13
|
作者：
Park, Jong-Min [1 ]
Kim, Moon-Ju [1 ]
Noh, Joo-Yoon [1 ]
Yun, Tae Gyeong [1 ]
Kang, Min-Jung [2 ]
Lee, Sang-Guk [3 ]
Yoo, Byong Chul [4 ]
Pyun, Jae-Chul [1 ]
机构：
[1] Yonsei Univ, Dept Mat Sci & Engn, Seoul 03722, South Korea
[2] Korea Inst Sci & Technol, Seoul 02792, South Korea
[3] Yonsei Univ, Severance Hosp, Dept Lab Med, Coll Med, Seoul 03722, South Korea
[4] Natl Canc Ctr, Res Inst, Biomarker Branch, Goyang 10408, South Korea
来源：
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2020年 / 31卷 / 04期
基金：
新加坡国家研究基金会;
关键词：
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; parylene-matrix chip; cancer biomarker; N-methyl-2-pyridone-5-carboxamide; glutamine; lysophosphatidylcholine; LASER-DESORPTION-IONIZATION; SYNTHESIZED TIO2 NANOWIRES; QUANTITATIVE-ANALYSIS; SUSCEPTIBILITY TEST; SOLID-MATRIX; DESORPTION/IONIZATION; IMMOBILIZATION; MOLECULES; PROTEINS; ASSAY;
D O I：
10.1021/jasms.9b00102
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Recently, the parylene-matrix chip was developed for quantitative analysis of small molecules less than 1 kDa. In this study, MALDI-TOF MS based on the parylene-matrix chip was performed to clinically diagnose intrahepatic cholangiocarcinoma (IHCC) and colorectal cancer (CRC). The parylene-matrix chip was applied for the detection of small cancer biomarkers, including N-methyl-2-pyridone-5-carboxamide (2PY), glutamine, lysophosphatidylcholine (LPC) 16:0, and LPC 18:0. The feasibility of MALDI-TOF MS based on the parylene-matrix chip was confirmed via analysis of spot-to-spot and shot-to-shot reproducibility. Serum metabolite markers of IHCC, N-methyl-2-pyridone-5-carboxamide (2PY), and glutamine were quantified using MALDI-TOF MS based on the parylene-matrix chip. For clinical diagnosis of CRC, two water-insoluble (barely soluble) biomarkers, lysophosphatidylcholine (LPC) 16:0 and LPC 18:0, were quantified. Finally, glutamine and LPC 16:0 were simultaneously detected at a range of concentrations in sera from colon cancer patients using the parylene-matrix chip. Thus, this method yielded highthroughput detection of cancer biomarkers for the mixture samples of water-soluble analytes (2PY and glutamine) and waterinsoluble analytes (LPC 16:0 and LPC 18:0).
引用
收藏
页码：917 / 926
页数：10
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