Simultaneous Analysis of Multiple Cancer Biomarkers Using MALDI-TOF Mass Spectrometry Based on a Parylene-Matrix Chip

被引：13
|
作者：
Park, Jong-Min [1 ]
Kim, Moon-Ju [1 ]
Noh, Joo-Yoon [1 ]
Yun, Tae Gyeong [1 ]
Kang, Min-Jung [2 ]
Lee, Sang-Guk [3 ]
Yoo, Byong Chul [4 ]
Pyun, Jae-Chul [1 ]
机构：
[1] Yonsei Univ, Dept Mat Sci & Engn, Seoul 03722, South Korea
[2] Korea Inst Sci & Technol, Seoul 02792, South Korea
[3] Yonsei Univ, Severance Hosp, Dept Lab Med, Coll Med, Seoul 03722, South Korea
[4] Natl Canc Ctr, Res Inst, Biomarker Branch, Goyang 10408, South Korea
来源：
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2020年 / 31卷 / 04期
基金：
新加坡国家研究基金会;
关键词：
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; parylene-matrix chip; cancer biomarker; N-methyl-2-pyridone-5-carboxamide; glutamine; lysophosphatidylcholine; LASER-DESORPTION-IONIZATION; SYNTHESIZED TIO2 NANOWIRES; QUANTITATIVE-ANALYSIS; SUSCEPTIBILITY TEST; SOLID-MATRIX; DESORPTION/IONIZATION; IMMOBILIZATION; MOLECULES; PROTEINS; ASSAY;
D O I：
10.1021/jasms.9b00102
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Recently, the parylene-matrix chip was developed for quantitative analysis of small molecules less than 1 kDa. In this study, MALDI-TOF MS based on the parylene-matrix chip was performed to clinically diagnose intrahepatic cholangiocarcinoma (IHCC) and colorectal cancer (CRC). The parylene-matrix chip was applied for the detection of small cancer biomarkers, including N-methyl-2-pyridone-5-carboxamide (2PY), glutamine, lysophosphatidylcholine (LPC) 16:0, and LPC 18:0. The feasibility of MALDI-TOF MS based on the parylene-matrix chip was confirmed via analysis of spot-to-spot and shot-to-shot reproducibility. Serum metabolite markers of IHCC, N-methyl-2-pyridone-5-carboxamide (2PY), and glutamine were quantified using MALDI-TOF MS based on the parylene-matrix chip. For clinical diagnosis of CRC, two water-insoluble (barely soluble) biomarkers, lysophosphatidylcholine (LPC) 16:0 and LPC 18:0, were quantified. Finally, glutamine and LPC 16:0 were simultaneously detected at a range of concentrations in sera from colon cancer patients using the parylene-matrix chip. Thus, this method yielded highthroughput detection of cancer biomarkers for the mixture samples of water-soluble analytes (2PY and glutamine) and waterinsoluble analytes (LPC 16:0 and LPC 18:0).
引用
收藏
页码：917 / 926
页数：10
相关论文
共 50 条
  • [31] Salivary proteomics of canine oral tumors using MALDI-TOF mass spectrometry and LC-tandem mass spectrometry
    Ploypetch, Sekkarin
    Roytrakul, Sittiruk
    Jaresitthikunchai, Janthima
    Phaonakrop, Narumon
    Krobthong, Sucheewin
    Suriyaphol, Gunnaporn
    PLOS ONE, 2019, 14 (07):
  • [32] DNA-Mediated rolling circle amplification for ultrasensitive detection of thrombin using MALDI-TOF mass spectrometry
    Wu, Zengnan
    Lin, Ling
    Khan, Mashooq
    Zhang, Weifei
    Mao, Sifeng
    Zheng, Yajing
    Li, Zenghe
    Lin, Jin-Ming
    CHEMICAL COMMUNICATIONS, 2018, 54 (82) : 11546 - 11549
  • [33] Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
    Schowe, Shawn W.
    Langeberg, Conner J.
    Chapman, Erich G.
    Brown, Kitty
    Resendiz, Marino J. E.
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2022, (182):
  • [34] The identification of synthetic homopolymer end groups and verification of their transformations using MALDI-TOF mass spectrometry
    Li, Yejia
    Hoskins, Jessica N.
    Sreerama, Subramanya G.
    Grayson, Michael A.
    Grayson, Scott M.
    JOURNAL OF MASS SPECTROMETRY, 2010, 45 (06): : 587 - 611
  • [35] Intact cell MALDI-TOF mass spectrometry-based approaches for the diagnosis of bloodstream infections
    La Scola, Bernard
    EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2011, 11 (03) : 287 - 298
  • [36] Functional mass nanoprobes inserted on live cells for in situ monitoring multiple secreted enzymes with MALDI-TOF mass spectrometry
    Feng, Nan
    Li, Yiran
    Sun, Jiahui
    Wang, Haiqi
    Ma, Qiulin
    Guo, Jingxing
    Ju, Huangxian
    NANO TODAY, 2023, 50
  • [37] Application of ionic liquid matrices in spectral analysis of poly(lactide) - solid state NMR spectroscopy versus matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry
    Miksa, Beata
    Sochacki, Marek
    Libiszowski, Jan
    Duda, Andrzej
    Ciesielski, Wlodzimierz
    Potrzebowski, Marek J.
    ANALYTICAL METHODS, 2012, 4 (02) : 377 - 383
  • [38] Rapid differentiation of new isolates with MALDI-TOF mass spectrometry via discriminant function analysis based on principal components
    Sariyar-Akbulut, Berna
    KOREAN JOURNAL OF CHEMICAL ENGINEERING, 2009, 26 (06) : 1645 - 1651
  • [39] Rapid identification and characterization of Vibrio species using whole-cell MALDI-TOF mass spectrometry
    Dieckmann, R.
    Strauch, E.
    Alter, T.
    JOURNAL OF APPLIED MICROBIOLOGY, 2010, 109 (01) : 199 - 211
  • [40] Cross-Contamination From Vial Caps for SEC Analysis Determined by MALDI-TOF Mass Spectrometry
    Veloso, A.
    Leiza, J. R.
    JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2017, 55 (02) : 137 - 141
12345