Simultaneous Analysis of Multiple Cancer Biomarkers Using MALDI-TOF Mass Spectrometry Based on a Parylene-Matrix Chip

被引：13
|
作者：
Park, Jong-Min [1 ]
Kim, Moon-Ju [1 ]
Noh, Joo-Yoon [1 ]
Yun, Tae Gyeong [1 ]
Kang, Min-Jung [2 ]
Lee, Sang-Guk [3 ]
Yoo, Byong Chul [4 ]
Pyun, Jae-Chul [1 ]
机构：
[1] Yonsei Univ, Dept Mat Sci & Engn, Seoul 03722, South Korea
[2] Korea Inst Sci & Technol, Seoul 02792, South Korea
[3] Yonsei Univ, Severance Hosp, Dept Lab Med, Coll Med, Seoul 03722, South Korea
[4] Natl Canc Ctr, Res Inst, Biomarker Branch, Goyang 10408, South Korea
来源：
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2020年 / 31卷 / 04期
基金：
新加坡国家研究基金会;
关键词：
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; parylene-matrix chip; cancer biomarker; N-methyl-2-pyridone-5-carboxamide; glutamine; lysophosphatidylcholine; LASER-DESORPTION-IONIZATION; SYNTHESIZED TIO2 NANOWIRES; QUANTITATIVE-ANALYSIS; SUSCEPTIBILITY TEST; SOLID-MATRIX; DESORPTION/IONIZATION; IMMOBILIZATION; MOLECULES; PROTEINS; ASSAY;
D O I：
10.1021/jasms.9b00102
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Recently, the parylene-matrix chip was developed for quantitative analysis of small molecules less than 1 kDa. In this study, MALDI-TOF MS based on the parylene-matrix chip was performed to clinically diagnose intrahepatic cholangiocarcinoma (IHCC) and colorectal cancer (CRC). The parylene-matrix chip was applied for the detection of small cancer biomarkers, including N-methyl-2-pyridone-5-carboxamide (2PY), glutamine, lysophosphatidylcholine (LPC) 16:0, and LPC 18:0. The feasibility of MALDI-TOF MS based on the parylene-matrix chip was confirmed via analysis of spot-to-spot and shot-to-shot reproducibility. Serum metabolite markers of IHCC, N-methyl-2-pyridone-5-carboxamide (2PY), and glutamine were quantified using MALDI-TOF MS based on the parylene-matrix chip. For clinical diagnosis of CRC, two water-insoluble (barely soluble) biomarkers, lysophosphatidylcholine (LPC) 16:0 and LPC 18:0, were quantified. Finally, glutamine and LPC 16:0 were simultaneously detected at a range of concentrations in sera from colon cancer patients using the parylene-matrix chip. Thus, this method yielded highthroughput detection of cancer biomarkers for the mixture samples of water-soluble analytes (2PY and glutamine) and waterinsoluble analytes (LPC 16:0 and LPC 18:0).
引用
收藏
页码：917 / 926
页数：10
相关论文
共 50 条
  • [21] Improved MALDI-TOF Microbial Mass Spectrometry Imaging by Application of a Dispersed Solid Matrix
    Vergeiner, Stefan
    Schafferer, Lukas
    Haas, Hubertus
    Mueller, Thomas
    JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2014, 25 (08) : 1498 - 1501
  • [22] Analysis of benzylpenicillin in milk using MALDI-TOF mass spectrometry with top-down synthesized TiO2 nanowires as the solid matrix
    Kim, Jo-Il
    Park, Jong-Min
    Noh, Joo-Yoon
    Hwang, Seong-Ju
    Kang, Min-Jung
    Pyun, Jae-Chul
    CHEMOSPHERE, 2016, 143 : 64 - 70
  • [23] Rapid diagnosis of periodontitis, a feasibility study using MALDI-TOF mass spectrometry
    Antezack, Angeline
    Chaudet, Herve
    Tissot-Dupont, Herve
    Brouqui, Philippe
    Monnet-Corti, Virginie
    PLOS ONE, 2020, 15 (03):
  • [24] Rapid peptidomic profiling of peritoneal fluid by MALDI-TOF mass spectrometry for the identification of biomarkers of endometriosis
    Siciliano, Rosa Anna
    Mazzeo, Maria Fiorella
    Spada, Valentina
    Facchiano, Angelo
    d'Acierno, Antonio
    Stocchero, Matteo
    De Franciscis, Pasquale
    Colacurci, Nicola
    Sannolo, Nicola
    Miraglia, Nadia
    GYNECOLOGICAL ENDOCRINOLOGY, 2014, 30 (12) : 872 - 876
  • [25] Quantification of nitroaromatic explosives in contaminated soil using MALDI-TOF mass spectrometry
    Kober, S. Liane
    Hollert, Henner
    Frohme, Marcus
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2019, 411 (23) : 5993 - 6003
  • [26] Quantitative Analysis of Cashmere and Other Animal Hair Fibers in Textiles Using MALDI-TOF Mass Spectrometry
    Ohashi, Shinichi
    Demura, Yuka
    Sano, Motoaki
    Yoshioka, Yoichiro
    SEN-I GAKKAISHI, 2014, 70 (06) : 114 - 120
  • [27] Ultrasensitive detection of protein biomarkers by MALDI-TOF mass spectrometry based on ZnFe2O4 nanoparticles and mass tagging signal amplification
    Zhu, Yan-Li
    Lian, Yan-Mei
    Wang, Ji-Kai
    Chen, Zeng-Ping
    Yu, Ru-Qin
    TALANTA, 2021, 224 (224)
  • [28] MALDI-TOF mass spectrometry technology for detecting biomarkers of antimicrobial resistance: current achievements and future perspectives
    Vrioni, Georgia
    Tsiamis, Constantinos
    Oikonomidis, George
    Theodoridou, Kalliopi
    Kapsimali, Violeta
    Tsakris, Athanasios
    ANNALS OF TRANSLATIONAL MEDICINE, 2018, 6 (12)
  • [29] MALDI-TOF Mass Spectrometry Based Identification of Brucella abortus in Bovines of Western Rajasthan
    Priyanka
    Shringi, Brij Nandan
    Choudhary, Om Prakash
    Kashyap, Sudhir Kumar
    INTERNATIONAL JOURNAL OF APPLIED RESEARCH IN VETERINARY MEDICINE, 2019, 17 (02): : 108 - 120
  • [30] Matrix Selection for the Visualization of Small Molecules and Lipids in Brain Tumors Using Untargeted MALDI-TOF Mass Spectrometry Imaging
    Lu, Tianyao
    Freytag, Lutz
    Narayana, Vinod K.
    Moore, Zachery
    Oliver, Shannon J.
    Valkovic, Adam
    Nijagal, Brunda
    Peterson, Amanda L.
    de Souza, David P.
    McConville, Malcolm J.
    Whittle, James R.
    Best, Sarah A.
    Freytag, Saskia
    METABOLITES, 2023, 13 (11)
12345