Preparation and Characterization of Double-Stranded DNA Brushes via Surface-Initiated Enzymatic Polymerization

被引：3

作者：

Mitomo, Hideyuki ^{[1
,2
]}

Nakamura, Satoshi ^{[3
]}

Suzuki, Yasunobu ^{[3
]}

Matsuo, Yasutaka ^{[1
]}

Niikura, Kenichi ^{[1
,2
]}

Ijiro, Kuniharu ^{[1
,2
]}

机构：

[1] Hokkaido Univ, Res Inst Elect Sci, Kita Ku, Kita 21,Nishi 10, Sapporo, Hokkaido 0010021, Japan

[2] Hokkaido Univ, Global Stn Soft Matter, Global Inst Collaborat Res & Educ, Kita Ku, Kita 21,Nishi 11, Sapporo, Hokkaido 0010021, Japan

[3] Hokkaido Univ, Grad Sch Chem Sci & Engn, Kita Ku, Kita 13,Nishi 8, Sapporo, Hokkaido 0608628, Japan

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2017年 / 17卷 / 12期

关键词：

DNA; Polymer Brush; Surface-Initiated Polymerization; Patterning; Conformation Change; PROTEIN; NANOSTRUCTURES; FABRICATION; MICROARRAYS; POLYMERASE; BIOLOGY; GROWTH; ARRAYS;

D O I：

10.1166/jnn.2017.14367

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this study, we have prepared double-stranded DNA brushes on glass or quartz substrates through the immobilization of biotinylated oligo-DNA, followed by hybridization and polymerization of the DNA by a surface-initiated enzymatic polymerization method. The density of the DNA brush was calculated based on UV spectra and agarose gel electrophoresis. Prepared DNA brushes showed a relatively high density of DNA chains (over 1500 chains/mu m(2)) independent of the length of the DNA. Atomic force microscopy of the 2D-patterned DNA brushes indicated dynamic changes in height under various salt concentrations. These results provided insight into the conformation of DNA brushes.

引用

页码：8995 / 9001

页数：7

共 40 条

[1] Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle Amplification [J].