The emerging process of Top Down mass spectrometry for protein analysis: biomarkers, protein-therapeutics, and achieving high throughput

被引：74

作者：

Kellie, John F. ^{[1
]}

Tran, John C. ^{[1
]}

Lee, Ji Eun ^{[1
]}

Ahlf, Dorothy R. ^{[1
]}

Thomas, Haylee M. ^{[1
]}

Ntai, Ioanna ^{[1
]}

Catherman, Adam D. ^{[1
]}

Durbin, Kenneth R. ^{[1
]}

Zamdborg, Leonid ^{[1
]}

Vellaichamy, Adaikkalam ^{[1
]}

Thomas, Paul M. ^{[1
]}

Kelleher, Neil L. ^{[1
]}

机构：

[1] Univ Illinois, Technol Dev Team, Ctr Top Down Prote, Urbana, IL 61801 USA

来源：

MOLECULAR BIOSYSTEMS | 2010年 / 6卷 / 09期

关键词：

PHASE LIQUID-CHROMATOGRAPHY; QUADRUPOLE ION-TRAP; INTACT PROTEINS; ELECTROSPRAY-IONIZATION; DISSOCIATION; IDENTIFICATION; PROTEOMICS; BOTTOM; ELECTROPHORESIS; QUANTIFICATION;

D O I：

10.1039/c000896f

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Top Down mass spectrometry (MS) has emerged as an alternative to common Bottom Up strategies for protein analysis. In the Top Down approach, intact proteins are fragmented directly in the mass spectrometer to achieve both protein identification and characterization, even capturing information on combinatorial post-translational modifications. Just in the past two years, Top Down MS has seen incremental advances in instrumentation and dedicated software, and has also experienced a major boost from refined separations of whole proteins in complex mixtures that have both high recovery and reproducibility. Combined with steadily advancing commercial MS instrumentation and data processing, a high-throughput workflow covering intact proteins and polypeptides up to 70 kDa is directly visible in the near future.

引用

页码：1532 / 1539

页数：8

共 57 条

[21] Fragmentation of multiply-charged intact protein ions using MALDI TOF-TOF mass spectrometry [J].

Liu, Zhaoyang ;

Schey, Kevin L. .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2008, 19 (02) :231-238

[22] Two-dimensional liquid separations-mass mapping of proteins from human cancer cell lysates [J].

Lubman, DM ;

Kachman, MT ;

Wang, HX ;

Gong, SY ;

Yan, F ;

Hamler, RL ;

O'Neil, KA ;

Zhu, K ;

Buchanan, NS ;

Barder, TJ .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 782 (1-2) :183-196

[23] Top-down protein sequencing and MS3 on a hybrid linear quadrupole ion trap-orbitrap mass spectrometer [J].

Macek, B ;

Waanders, LF ;

Olsen, JV ;

Mann, M .

MOLECULAR & CELLULAR PROTEOMICS, 2006, 5 (05) :949-958

[24] Performance evaluation of a hybrid linear ion trap/orbitrap mass spectrometer [J].

Makarov, A ;

Denisov, E ;

Kholomeev, A ;

Baischun, W ;

Lange, O ;

Strupat, K ;

Horning, S .

ANALYTICAL CHEMISTRY, 2006, 78 (07) :2113-2120

[25]

MARSHALL KK, 1998, AGBIOFORUM, V1, P35

[26] Molecular-level description of proteins from Saccharomyces cerevisiae using quadrupole FT hybrid mass spectrometry for top down proteomics [J].

Meng, FY ;

Du, Y ;

Miller, LM ;

Patrie, SM ;

Robinson, DE ;

Kelleher, NL .

ANALYTICAL CHEMISTRY, 2004, 76 (10) :2852-2858

[27] Protein fractionation in a multicompartment device using Off-Gel™ isoelectric focusing [J].

Michel, PE ;

Reymond, F ;

Arnaud, IL ;

Josserand, J ;

Girault, HH ;

Rossier, JS .

ELECTROPHORESIS, 2003, 24 (1-2) :3-11

[28] Free-flow electrophoresis for top-down proteomics by Fourier transform ion cyclotron resonance mass spectrometry [J].

Ouvry-Patat, Severine A. ;

Torres, Matthew P. ;

Quek, Hung-Hiang ;

Gelfand, Craig A. ;

O'Mullan, Patrick ;

Nissum, Mikkel ;

Schroeder, Gottfried K. ;

Han, Jun ;

Elliott, Monica ;

Dryhurst, Deanna ;

Ausio, Juan ;

Wolfenden, Richard ;

Borchers, Christoph H. .

PROTEOMICS, 2008, 8 (14) :2798-2808

[29]

Perkins DN, 1999, ELECTROPHORESIS, V20, P3551, DOI 10.1002/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO

[30]

2-2

← 1 2 3 4 5 6 →