The emerging process of Top Down mass spectrometry for protein analysis: biomarkers, protein-therapeutics, and achieving high throughput

被引：74

作者：

Kellie, John F. ^{[1
]}

Tran, John C. ^{[1
]}

Lee, Ji Eun ^{[1
]}

Ahlf, Dorothy R. ^{[1
]}

Thomas, Haylee M. ^{[1
]}

Ntai, Ioanna ^{[1
]}

Catherman, Adam D. ^{[1
]}

Durbin, Kenneth R. ^{[1
]}

Zamdborg, Leonid ^{[1
]}

Vellaichamy, Adaikkalam ^{[1
]}

Thomas, Paul M. ^{[1
]}

Kelleher, Neil L. ^{[1
]}

机构：

[1] Univ Illinois, Technol Dev Team, Ctr Top Down Prote, Urbana, IL 61801 USA

来源：

MOLECULAR BIOSYSTEMS | 2010年 / 6卷 / 09期

关键词：

PHASE LIQUID-CHROMATOGRAPHY; QUADRUPOLE ION-TRAP; INTACT PROTEINS; ELECTROSPRAY-IONIZATION; DISSOCIATION; IDENTIFICATION; PROTEOMICS; BOTTOM; ELECTROPHORESIS; QUANTIFICATION;

D O I：

10.1039/c000896f

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Top Down mass spectrometry (MS) has emerged as an alternative to common Bottom Up strategies for protein analysis. In the Top Down approach, intact proteins are fragmented directly in the mass spectrometer to achieve both protein identification and characterization, even capturing information on combinatorial post-translational modifications. Just in the past two years, Top Down MS has seen incremental advances in instrumentation and dedicated software, and has also experienced a major boost from refined separations of whole proteins in complex mixtures that have both high recovery and reproducibility. Combined with steadily advancing commercial MS instrumentation and data processing, a high-throughput workflow covering intact proteins and polypeptides up to 70 kDa is directly visible in the near future.

引用

页码：1532 / 1539

页数：8

共 57 条

[1] Whole protein dissociation in a quadrupole ion trap: Identification of an a priori unknown modified protein [J].

Amunugama, R ;

Hogan, JM ;

Newton, KA ;

McLuckey, SA .

ANALYTICAL CHEMISTRY, 2004, 76 (03) :720-727

[2] Top-down proteomics with a quadrupole time-of-flight mass spectrometer and collision-induced dissociation [J].

Armirotti, Andrea ;

Benatti, Umberto ;

Damonte, Gianluca .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2009, 23 (05) :661-666

[3] In Vivo Phosphorylation Site Mapping in Mouse Cardiac Troponin I by High Resolution Top-Down Electron Capture Dissociation Mass Spectrometry: Ser22/23 Are the Only Sites Basally Phosphorylated [J].

Ayaz-Guner, Serife ;

Zhang, Jiang ;

Li, Lin ;

Walker, Jeffery W. ;

Ge, Ying .

BIOCHEMISTRY, 2009, 48 (34) :8161-8170

[4] Automated proteomics of E-coli via top-down electron-transfer dissociation mass spectrometry [J].

Bunger, Maureen K. ;

Cargile, Benjamin J. ;

Ngunjiri, Anne ;

Bundy, Jonathan L. ;

Stephenson, James L., Jr. .

ANALYTICAL CHEMISTRY, 2008, 80 (05) :1459-1467

[5] Analysis of intact proteins on a chromatographic time scale by electron transfer dissociation tandem mass spectrometry [J].

Chi, An ;

Bai, Dina L. ;

Geer, Lewis Y. ;

Shabanowitz, Jeffrey ;

Hunt, Donald F. .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2007, 259 (1-3) :197-203

[6] Top-down identification and quantification of stable isotope labeled proteins from Aspergillus flavus using online nano-flow reversed-phase liquid chromatography coupled to a LTQ-FTICR mass spectrometer [J].

Collier, Timothy S. ;

Hawkridge, Adam M. ;

Georgianna, D. Ryan ;

Payne, Gary A. ;

Muddiman, David C. .

ANALYTICAL CHEMISTRY, 2008, 80 (13) :4994-5001

[7]

COMPTON PD, UNPUB

[8] Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast [J].

de Godoy, Lyris M. F. ;

Olsen, Jesper V. ;

Cox, Juergen ;

Nielsen, Michael L. ;

Hubner, Nina C. ;

Froehlich, Florian ;

Walther, Tobias C. ;

Mann, Matthias .

NATURE, 2008, 455 (7217) :1251-U60

[9] Top-down and bottom-up mass spectrometric characterization of human myoglobin-centered free radicals induced by oxidative damage [J].

Deterding, Leesa J. ;

Lhattacharjee, Suchandra ;

Ramirez, Dario C. ;

Mason, Ronald P. ;

Tomer, Kenneth B. .

ANALYTICAL CHEMISTRY, 2007, 79 (16) :6236-6248

[10] Top-Down Lipidomics Reveals Ether Lipid Deficiency in Blood Plasma of Hypertensive Patients [J].

Graessler, Juergen ;

Schwudke, Dominik ;

Schwarz, Peter E. H. ;

Herzog, Ronny ;

Shevchenko, Andrej ;

Bornstein, Stefan R. .

PLOS ONE, 2009, 4 (07)

← 1 2 3 4 5 6 →