SEPT9 and SHOX2 DNA methylation status and its utility in the diagnosis of colonic adenomas and colorectal adenocarcinomas

被引：48

作者：

Semaan, Alexander ^{[1
]}

van Ellen, Anne ^{[2
]}

Meller, Sebastian ^{[2
]}

Bergheim, Dominik ^{[2
]}

Branchi, Vittorio ^{[1
]}

Lingohr, Philipp ^{[1
]}

Goltz, Diane ^{[2
]}

Kalff, Joerg C. ^{[1
]}

Kristiansen, Glen ^{[2
]}

Matthaei, Hanno ^{[1
]}

Pantelis, Dimitrios ^{[1
]}

Dietrich, Dimo ^{[2
]}

机构：

[1] Univ Bonn, Dept Gen Visceral Thorac & Vasc Surg, Sigmund Freud Str 25, D-53127 Bonn, Germany

[2] Univ Bonn, Inst Pathol, Sigmund Freud Str 25, D-53127 Bonn, Germany

来源：

CLINICAL EPIGENETICS | 2016年 / 8卷

关键词：

SHOX2; SEPT9; Adenomas; Colorectal cancer; Methylation; Amplification; LUNG-CANCER; PERIPHERAL-BLOOD; PERFORMANCE; ASSAY; BIOMARKERS; PLASMA; GENE; CLASSIFICATION; COLONOSCOPY; EPIGENOME;

D O I：

10.1186/s13148-016-0267-5

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Colorectal cancer (CRC) appear to arise from precursor lesions in a well-characterized adenoma-carcinoma sequence. Significant efforts have been invested to develop biomarkers that identify early adenocarcinomas and adenomas with high-grade dysplasia, since these are believed to harbor a particularly high risk for malignant transition and thus require resection. Promoter methylation of SEPT9 and SHOX2 has been suggested as a biomarker for various solid malignant tumors. Hence, the present study aimed to test their biomarker potential in CRC and precursor lesions. Results: Assessment of promoter methylation of SEPT9 distinguished adenomas and CRC from controls as well as advanced from non-advanced adenomas (all p < 0.001). Correspondingly, SHOX2 methylation levels in adenomas and colorectal carcinomas were significantly higher compared to those in normal control tissues (p < 0.001). Histologic transition from adenomas to CRC was paralleled by amplification of the SEPT9 gene locus. Conclusions: SEPT9/SHOX2 methylation assays may help to distinguish colorectal cancer and adenomas from normal and inflammatory colonic tissue, as well as advanced from non-advanced adenomas. Further studies need to validate these findings before introduction in clinical routine.

引用

页数：10

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