The cathelicidin-like peptide derived from panda genome is a potential antimicrobial peptide

被引：9

作者：

Yan, Xiuwen ^{[1
]}

Zhong, Jian ^{[3
]}

Liu, Huan ^{[2
,4
]}

Liu, Cunbao ^{[2
,4
]}

Zhang, Keyun ^{[1
]}

Lai, Ren ^{[1
,2
]}

机构：

[1] Nanjing Agr Univ, Life Sci Coll, Nanjing 210095, Jiangsu, Peoples R China

[2] Chinese Acad Sci, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Kunming 650223, Yunnan, Peoples R China

[3] Nanjing Maternal & Child Hlth Hosp, Nanjing 210004, Jiangsu, Peoples R China

[4] Chinese Acad Sci, Grad Sch, Beijing 100009, Peoples R China

来源：

GENE | 2012年 / 492卷 / 02期

关键词：

Cathelicidic; Antimicrobial peptides; Panda; Innate immunity; HOST-DEFENSE PEPTIDES; STRUCTURAL FEATURES; INNATE IMMUNITY; SKIN; ANTIBIOTICS; FROG; LEUKOCYTES; INHIBITOR; FAMILIES; SEQUENCE;

D O I：

10.1016/j.gene.2011.11.009

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A novel cathelicidin-like antimicrobial peptide was identified by mining genome of panda. This peptide (cathelicidin-AM) was synthesized. It showed potential antimicrobial activities against wide spectrum of microorganisms including Gram-negative and -positive bacteria, and fungi. It had similar antimicrobial abilities against both standard and clinically isolated drug-resistant strains. Cathelicidin-AM could rapidly exert its antibacterial activities. It just took less than I h to kill all Staphylococcus sciuri at the concentration of 2, 4 or 10 times of minimal inhibitory concentration (MIC) while clindamycin took 6 h. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analysis indicated that cathelicidin-AM killed bacteria by directly affecting bacterial cell wall and membrane. Phylogenetic analysis revealed that the panda cathelicidin had the nearest evolution relationship with dog cathelicidin. The current work provides a novel cathelicidin-like peptide with strong antimicrobial abilities. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：368 / 374

页数：7

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