Detection of Chronic Wasting Disease Prions in Fetal Tissues of Free-Ranging White-Tailed Deer

被引:14
作者
Nalls, Amy V. [1 ]
McNulty, Erin E. [1 ]
Mayfield, Amber [1 ]
Crum, James M. [2 ]
Keel, Michael K. [3 ]
Hoover, Edward A. [1 ]
Ruder, Mark G. [4 ]
Mathiason, Candace K. [1 ]
机构
[1] Colorado State Univ, Dept Microbiol Immunol & Pathol, Coll Vet Med & Biomed Sci, Ft Collins, CO 80523 USA
[2] West Virginia Div Nat Resources, Wildlife Resources Sect, Elkins, WV 26241 USA
[3] Univ Calif Davis, Sch Vet Med, Dept Pathol Microbiol & Immunol, Davis, CA 95616 USA
[4] Univ Georgia, Coll Vet Med, Southeastern Cooperat Wildlife Dis Study, Athens, GA 30602 USA
来源
VIRUSES-BASEL | 2021年 / 13卷 / 12期
基金
美国国家卫生研究院;
关键词
prions; chronic wasting disease; mother-to-offspring transmission; sPMCA; RT-QuIC; fetal tissues; IN-UTERO TRANSMISSION; PRPSC ACCUMULATION; BINUCLEATE CELL; SYNEPITHELIOCHORIAL PLACENTA; CLASSICAL SCRAPIE; BLOOD-TRANSFUSION; NATURAL SCRAPIE; SHEEP; INFECTIVITY; GENETICS;
D O I
10.3390/v13122430
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The transmission of chronic wasting disease (CWD) has largely been attributed to contact with infectious prions shed in excretions (saliva, urine, feces, blood) by direct animal-to-animal exposure or indirect contact with the environment. Less-well studied has been the role that mother-to-offspring transmission may play in the facile transmission of CWD, and whether mother-to-offspring transmission before birth may contribute to the extensive spread of CWD. We thereby focused on a population of free-ranging white-tailed deer from West Virginia, USA, in which CWD has been detected. Fetal tissues, ranging from 113 to 158 days of gestation, were harvested from the uteri of CWD+ dams in the asymptomatic phase of infection. Using serial protein misfolding amplification (sPMCA), we detected evidence of prion seeds in 7 of 14 fetuses (50%) from 7 of 9 pregnancies (78%), with the earliest detection at 113 gestational days. This is the first report of CWD detection in free ranging white-tailed deer fetal tissues. Further investigation within cervid populations across North America will help define the role and impact of mother-to-offspring vertical transmission of CWD.
引用
收藏
页数:11
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