Detection of Chronic Wasting Disease Prions in Fetal Tissues of Free-Ranging White-Tailed Deer

被引:12
作者
Nalls, Amy V. [1 ]
McNulty, Erin E. [1 ]
Mayfield, Amber [1 ]
Crum, James M. [2 ]
Keel, Michael K. [3 ]
Hoover, Edward A. [1 ]
Ruder, Mark G. [4 ]
Mathiason, Candace K. [1 ]
机构
[1] Colorado State Univ, Dept Microbiol Immunol & Pathol, Coll Vet Med & Biomed Sci, Ft Collins, CO 80523 USA
[2] West Virginia Div Nat Resources, Wildlife Resources Sect, Elkins, WV 26241 USA
[3] Univ Calif Davis, Sch Vet Med, Dept Pathol Microbiol & Immunol, Davis, CA 95616 USA
[4] Univ Georgia, Coll Vet Med, Southeastern Cooperat Wildlife Dis Study, Athens, GA 30602 USA
来源
VIRUSES-BASEL | 2021年 / 13卷 / 12期
基金
美国国家卫生研究院;
关键词
prions; chronic wasting disease; mother-to-offspring transmission; sPMCA; RT-QuIC; fetal tissues; IN-UTERO TRANSMISSION; PRPSC ACCUMULATION; BINUCLEATE CELL; SYNEPITHELIOCHORIAL PLACENTA; CLASSICAL SCRAPIE; BLOOD-TRANSFUSION; NATURAL SCRAPIE; SHEEP; INFECTIVITY; GENETICS;
D O I
10.3390/v13122430
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The transmission of chronic wasting disease (CWD) has largely been attributed to contact with infectious prions shed in excretions (saliva, urine, feces, blood) by direct animal-to-animal exposure or indirect contact with the environment. Less-well studied has been the role that mother-to-offspring transmission may play in the facile transmission of CWD, and whether mother-to-offspring transmission before birth may contribute to the extensive spread of CWD. We thereby focused on a population of free-ranging white-tailed deer from West Virginia, USA, in which CWD has been detected. Fetal tissues, ranging from 113 to 158 days of gestation, were harvested from the uteri of CWD+ dams in the asymptomatic phase of infection. Using serial protein misfolding amplification (sPMCA), we detected evidence of prion seeds in 7 of 14 fetuses (50%) from 7 of 9 pregnancies (78%), with the earliest detection at 113 gestational days. This is the first report of CWD detection in free ranging white-tailed deer fetal tissues. Further investigation within cervid populations across North America will help define the role and impact of mother-to-offspring vertical transmission of CWD.
引用
收藏
页数:11
相关论文
共 50 条
  • [21] Evaluating the ability of a locally focused culling program in removing chronic wasting disease infected free-ranging white-tailed deer in Illinois, USA, 2003-2020
    Varga, Csaba
    McDonald, Patrick
    Brown, William M.
    Shelton, Paul
    Roca, Alfred L.
    Novakofski, Jan E.
    Mateus-Pinilla, Nohra E.
    TRANSBOUNDARY AND EMERGING DISEASES, 2022, 69 (05) : 2867 - 2878
  • [22] Accurate Genomic Predictions for Chronic Wasting Disease in US White-Tailed Deer
    Seabury, Christopher M.
    Oldeschulte, David L.
    Bhattarai, Eric K.
    Legare, Dhruti
    Ferro, Pamela J.
    Metz, Richard P.
    Johnson, Charles D.
    Lockwood, Mitchell A.
    Nichols, Tracy A.
    G3-GENES GENOMES GENETICS, 2020, 10 (04): : 1433 - 1441
  • [23] RT-QuIC detection of chronic wasting disease prion in platelet samples of white-tailed deer
    Kobashigawa, Estela
    Russell, Sherri
    Zhang, Michael Z.
    Sinnott, Emily A.
    Connolly, Michael
    Zhang, Shuping
    BMC VETERINARY RESEARCH, 2024, 20 (01)
  • [24] Diagnostic testing of chronic wasting disease in white-tailed deer (Odocoileus virginianus) by RT-QuIC using multiple tissues
    Burgener, Kate R.
    Lichtenberg, Stuart S.
    Lomax, Aaron
    Storm, Daniel J.
    Walsh, Daniel P.
    Pedersen, Joel A.
    PLOS ONE, 2022, 17 (11):
  • [25] Infectious Prions in the Pregnancy Microenvironment of Chronic Wasting Disease-Infected Reeves' Muntjac Deer
    Nalls, Amy V.
    McNulty, Erin
    Hoover, Clare E.
    Pulscher, Laura A.
    Hoover, Edward A.
    Mathiason, Candace K.
    JOURNAL OF VIROLOGY, 2017, 91 (15)
  • [26] Movement of white-tailed deer in contrasting landscapes influences management of chronic wasting disease
    Jennelle, Christopher S.
    Walter, W. David
    Crawford, Joanne
    Rosenberry, Christopher S.
    Wallingford, Bret D.
    JOURNAL OF WILDLIFE MANAGEMENT, 2022, 86 (08)
  • [27] Surveillance and Monitoring of White-Tailed Deer for Chronic Wasting Disease in the Northeastern United States
    Evans, Tyler S.
    Schuler, Krysten L.
    Walter, W. David
    JOURNAL OF FISH AND WILDLIFE MANAGEMENT, 2014, 5 (02): : 387 - 393
  • [28] Chronic wasting disease in white-tailed deer: infection, mortality, and implications for heterogeneous transmission
    Samuel, Michael D.
    Storm, Daniel J.
    ECOLOGY, 2016, 97 (11) : 3195 - 3205
  • [29] Genotype by environment interactions for chronic wasting disease in farmed US white-tailed deer
    Seabury, Christopher M.
    Lockwood, Mitchell A.
    Nichols, Tracy A.
    G3-GENES GENOMES GENETICS, 2022, 12 (07):
  • [30] Successful transmission of the chronic wasting disease (CWD) agent to white-tailed deer by intravenous blood transfusion
    Mammadova, Najiba
    Cassmann, Eric
    Greenlee, Justin J.
    RESEARCH IN VETERINARY SCIENCE, 2020, 133 : 304 - 306