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Detection of Chronic Wasting Disease Prions in Fetal Tissues of Free-Ranging White-Tailed Deer
被引:12
|作者:
Nalls, Amy V.
[1
]
McNulty, Erin E.
[1
]
Mayfield, Amber
[1
]
Crum, James M.
[2
]
Keel, Michael K.
[3
]
Hoover, Edward A.
[1
]
Ruder, Mark G.
[4
]
Mathiason, Candace K.
[1
]
机构:
[1] Colorado State Univ, Dept Microbiol Immunol & Pathol, Coll Vet Med & Biomed Sci, Ft Collins, CO 80523 USA
[2] West Virginia Div Nat Resources, Wildlife Resources Sect, Elkins, WV 26241 USA
[3] Univ Calif Davis, Sch Vet Med, Dept Pathol Microbiol & Immunol, Davis, CA 95616 USA
[4] Univ Georgia, Coll Vet Med, Southeastern Cooperat Wildlife Dis Study, Athens, GA 30602 USA
来源:
VIRUSES-BASEL
|
2021年
/
13卷
/
12期
基金:
美国国家卫生研究院;
关键词:
prions;
chronic wasting disease;
mother-to-offspring transmission;
sPMCA;
RT-QuIC;
fetal tissues;
IN-UTERO TRANSMISSION;
PRPSC ACCUMULATION;
BINUCLEATE CELL;
SYNEPITHELIOCHORIAL PLACENTA;
CLASSICAL SCRAPIE;
BLOOD-TRANSFUSION;
NATURAL SCRAPIE;
SHEEP;
INFECTIVITY;
GENETICS;
D O I:
10.3390/v13122430
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
The transmission of chronic wasting disease (CWD) has largely been attributed to contact with infectious prions shed in excretions (saliva, urine, feces, blood) by direct animal-to-animal exposure or indirect contact with the environment. Less-well studied has been the role that mother-to-offspring transmission may play in the facile transmission of CWD, and whether mother-to-offspring transmission before birth may contribute to the extensive spread of CWD. We thereby focused on a population of free-ranging white-tailed deer from West Virginia, USA, in which CWD has been detected. Fetal tissues, ranging from 113 to 158 days of gestation, were harvested from the uteri of CWD+ dams in the asymptomatic phase of infection. Using serial protein misfolding amplification (sPMCA), we detected evidence of prion seeds in 7 of 14 fetuses (50%) from 7 of 9 pregnancies (78%), with the earliest detection at 113 gestational days. This is the first report of CWD detection in free ranging white-tailed deer fetal tissues. Further investigation within cervid populations across North America will help define the role and impact of mother-to-offspring vertical transmission of CWD.
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页数:11
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