Methods in cell separations

被引:61
作者
Dainiak, Maria B.
Kumar, Ashok
Galaev, Igor Yu.
Mattiasson, Bo
机构
[1] Lund Univ, Dept Biotechnol, Ctr Chem & Chem Engn, S-22100 Lund, Sweden
[2] Protista Biotechnol AB, IDEON, S-22370 Lund, Sweden
[3] Indian Inst Technol, Dept Biol Sci & Bioengn, Kanpur 208016, Uttar Pradesh, India
来源
CELL SEPARATION: FUNDAMENTALS, ANALYTICAL AND PREPARATIVE METHODS | 2007年 / 106卷
关键词
cell separation; purity; recovery; throughput; surface antigen; antibodies; stem cells;
D O I
10.1007/10_2007_069
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Research in the field of cell biology and biomedicine relies on technologies that fractionate cell populations and isolate rare cell types to high purity. A brief overview of methods and commercially available products currently used in cell separations is presented. Cell fractionation by size and density and highly selective affinity-based technologies such as affinity chromatography, fluorescence-activated cell sorting (FACS) and magnetic cell sorting are discussed in terms of throughput, yield, and purity.
引用
收藏
页码:1 / 18
页数:18
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