Methods in cell separations

被引：61

作者：

Dainiak, Maria B.

Kumar, Ashok

Galaev, Igor Yu.

Mattiasson, Bo

机构：

[1] Lund Univ, Dept Biotechnol, Ctr Chem & Chem Engn, S-22100 Lund, Sweden

[2] Protista Biotechnol AB, IDEON, S-22370 Lund, Sweden

[3] Indian Inst Technol, Dept Biol Sci & Bioengn, Kanpur 208016, Uttar Pradesh, India

来源：

CELL SEPARATION: FUNDAMENTALS, ANALYTICAL AND PREPARATIVE METHODS | 2007年 / 106卷

关键词：

cell separation; purity; recovery; throughput; surface antigen; antibodies; stem cells;

D O I：

10.1007/10_2007_069

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Research in the field of cell biology and biomedicine relies on technologies that fractionate cell populations and isolate rare cell types to high purity. A brief overview of methods and commercially available products currently used in cell separations is presented. Cell fractionation by size and density and highly selective affinity-based technologies such as affinity chromatography, fluorescence-activated cell sorting (FACS) and magnetic cell sorting are discussed in terms of throughput, yield, and purity.

引用

页码：1 / 18

页数：18

共 78 条

[41] Autologous transplantation of CD133 selected hematopoietic progenitor cells in a pediatric patient with relapsed leukemia [J].

Koehl, U ;

Zimmermann, S ;

Esser, R ;

Sörensen, J ;

Grüttner, HP ;

Duchscherer, M ;

Seifried, E ;

Klingebiel, T ;

Schwabe, D .

BONE MARROW TRANSPLANTATION, 2002, 29 (11) :927-930

[42]

Krause DS, 1996, BLOOD, V87, P1

[43] Affinity binding of cells to cryogel adsorbents with immobilized specific ligands:: effect of ligand coupling and matrix architecture [J].

Kumar, A ;

Rodríguez-Caballero, A ;

Plieva, FM ;

Galaev, IY ;

Nandakumar, KS ;

Kamihira, M ;

Holmdahl, R ;

Orfao, A ;

Mattiasson, B .

JOURNAL OF MOLECULAR RECOGNITION, 2005, 18 (01) :84-93

[44] Affinity fractionation of lymphocytes using a monolithic cryogel [J].

Kumar, A ;

Plieva, FM ;

Galeav, IY ;

Mattiasson, B .

JOURNAL OF IMMUNOLOGICAL METHODS, 2003, 283 (1-2) :185-194

[45] Transplantation of a combination of CD133+ and CD34+ selected progenitor cells from alternative donors [J].

Lang, P ;

Bader, P ;

Schumm, M ;

Feuchtinger, T ;

Einsele, H ;

Führer, M ;

Weinstock, C ;

Handgretinger, R ;

Kuci, S ;

Martin, D ;

Niethammer, D ;

Greil, J .

BRITISH JOURNAL OF HAEMATOLOGY, 2004, 124 (01) :72-79

[46]

Law P, 1993, J Hematother, V2, P247, DOI 10.1089/scd.1.1993.2.247

[47] Concomitant mobilization of plasma cells and hematopoietic progenitors into peripheral blood of multiple myeloma patients: Positive selection and transplantation of enriched CD34(+) cells to remove circulating tumor cells [J].

Lemoli, RM ;

Fortuna, A ;

Motta, MR ;

Rizzi, S ;

Giudice, V ;

Nannetti, A ;

Martinelli, G ;

Cavo, M ;

Amabile, M ;

Mangianti, S ;

Fogli, M ;

Conte, R ;

Tura, S .

BLOOD, 1996, 87 (04) :1625-1634

[48] Transplantation of allogeneic CD34(+) blood cells [J].

Link, H ;

Arseniev, L ;

Bahre, O ;

Kadar, JG ;

Diedrich, H ;

Poliwoda, H .

BLOOD, 1996, 87 (11) :4903-4909

[49] G-CSF alone mobilizes sufficient peripheral blood CD34(+) cells for positive selection in newly diagnosed patients with myeloma and lymphoma [J].

Mahe, B ;

Milpied, N ;

Hermouet, S ;

Robillard, N ;

Moreau, P ;

Letortorec, S ;

Rapp, MJ ;

Bataille, R ;

Harousseau, JL .

BRITISH JOURNAL OF HAEMATOLOGY, 1996, 92 (02) :263-268

[50]

Mammen M, 1998, ANGEW CHEM INT EDIT, V37, P2755

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