Methods in cell separations

被引：61

作者：

Dainiak, Maria B.

Kumar, Ashok

Galaev, Igor Yu.

Mattiasson, Bo

机构：

[1] Lund Univ, Dept Biotechnol, Ctr Chem & Chem Engn, S-22100 Lund, Sweden

[2] Protista Biotechnol AB, IDEON, S-22370 Lund, Sweden

[3] Indian Inst Technol, Dept Biol Sci & Bioengn, Kanpur 208016, Uttar Pradesh, India

来源：

CELL SEPARATION: FUNDAMENTALS, ANALYTICAL AND PREPARATIVE METHODS | 2007年 / 106卷

关键词：

cell separation; purity; recovery; throughput; surface antigen; antibodies; stem cells;

D O I：

10.1007/10_2007_069

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Research in the field of cell biology and biomedicine relies on technologies that fractionate cell populations and isolate rare cell types to high purity. A brief overview of methods and commercially available products currently used in cell separations is presented. Cell fractionation by size and density and highly selective affinity-based technologies such as affinity chromatography, fluorescence-activated cell sorting (FACS) and magnetic cell sorting are discussed in terms of throughput, yield, and purity.

引用

页码：1 / 18

页数：18

共 78 条

[1] Commercial high speed machines open new opportunities in high throughput flow cytometry (HTFC) [J].