Phytase produced on citric byproducts: purification and characterization

被引：20

作者：

Spier, M. R. ^{[1
]}

Fendrich, R. C. ^{[1
]}

Almeida, P. C. ^{[1
]}

Noseda, M. ^{[1
]}

Greiner, R. ^{[2
]}

Konietzny, U. ^{[2
]}

Woiciechowski, A. L. ^{[1
]}

Soccol, V. T. ^{[1
]}

Soccol, C. R. ^{[1
]}

机构：

[1] Fed Univ Parana UFPR, Bioproc Engn & Biotechnol Dept, BR-81531990 Curitiba, Parana, Brazil

[2] Max Rubner Inst, Dept Food & Bioproc Engn, Fed Res Inst Nutr & Food, D-76131 Karlsruhe, Germany

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2011年 / 27卷 / 02期

关键词：

Citric pulp; Chromatofocusing; Eletrophoresis; Phytase; Purification; Solid-state fermentation; ASPERGILLUS-NIGER PHYTASE; SOLID-STATE FERMENTATION; BIOCHEMICAL-CHARACTERIZATION; EXTRACELLULAR PHYTASE; FUMIGATUS PHYTASE; MICROBIAL PHYTASE; IN-VITRO; PHYA; EXPRESSION; PHOSPHATE;

D O I：

10.1007/s11274-010-0455-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Citric pulp is an agro-industrial residue from the citrus processing industry with low inorganic phosphorus content applied in animal feed. A new bioprocess was developed to produce and purify a new phytase generated on citric pulp fermentation by Aspergillus niger FS3. The phytase was purified by cationic-exchange, anionic-exchange chromatography and chromatofocusing steps. From SDS-PAGE analysis, the molecular weight of the purified phytase was calculated to be 108 kDa. The phytase had an optimum pH of 5.0-5.5 and an optimum temperature of 60A degrees C. The phytase displayed high affinity for phytate, and the K (m) was 0.52 mM. The purified phytase was sufficiently able to withstand pelleting temperatures, retaining sufficiently high phytate-degrading activity.

引用

页码：267 / 274

页数：8

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