Enzymatic activity and partial purification of solanapyrone synthase: first enzyme catalyzing Diels-Alder reaction

被引：79

作者：

Katayama, K ^{[1
]}

Kobayashi, T ^{[1
]}

Oikawa, H ^{[1
]}

Honma, M ^{[1
]}

Ichihara, A ^{[1
]}

机构：

[1] Hokkaido Univ, Fac Agr, Dept Biosci & Chem, Sapporo, Hokkaido 060, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1998年 / 1384卷 / 02期

关键词：

Diels-Alder reaction; alcohol oxidase; solanapyrone; Diels-Alderase; solanapyrone synthase; (Alternaria solani);

D O I：

10.1016/S0167-4838(98)00040-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In cell-foe extracts of Alternaria solani, an enzymatic activity converting prosolanapyrone II to solanapyrones A and D via oxidation and subsequent Diels-Alder reaction has been found. Chromatography with DEAE-Sepharose provided two active fractions, pools 1 and 2. The former fraction converted prosolanapyrone II to solanapyrones A and D in a ratio of 2.2:1 with optical purities of 99% and 45% ee, respectively. The latter fraction did so in a ratio of 7.6:1 with 99% and nearly 0% ee, respectively. The enzyme partially purified from pool 2 native molecular weight of 40-62 kD and a pi of 4.25. The high reactivity of prosolanapyrone III in aqueous solution and the chromatographic behavior of the enzyme in pool 2 suggest that a single enzyme catalyzes both the oxidation and Diels-Alder reaction. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：387 / 395

页数：9

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