Purification and characterisation of α-glucosidase inhibitory peptides from defatted camellia seed cake

被引：32

作者：

Feng, Jun ^{[1
]}

Ma, Yi-Long ^{[1
]}

Sun, Ping ^{[1
]}

Thakur, Kiran ^{[1
]}

Wang, Shaoyun ^{[2
]}

Zhang, Jian-Guo ^{[1
]}

Wei, Zhao-Jun ^{[1
,3
]}

机构：

[1] Hefei Univ Technol, Sch Food Sci & Biol Engn, Hefei 230009, Peoples R China

[2] Fuzhou Univ, Coll Biol Sci & Technol, Fuzhou 350108, Peoples R China

[3] North Minzu Univ, Biol Sci & Engn Coll, Yinchuan 750021, Ningxia, Peoples R China

来源：

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY | 2021年 / 56卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Camellia seed cake; protein hydrolysate; purified peptides; alpha-Glucosidase inhibitory activity; ANTIOXIDANT PEPTIDES; BIOACTIVE PEPTIDES; PROTEIN; IDENTIFICATION; HYDROLYSATE; CHROMATOGRAPHY; EXTRACTION; TRIPEPTIDE;

D O I：

10.1111/ijfs.14613

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Enzymatically derived peptides from different proteins have displayed the potential to provide health benefits. Present research aims to obtain the functional peptides and improve the economic value of camellia seed cake via enzymatic hydrolysis. Camellia seed cake protein hydrolysates (CSCPH) prepared with alcalase demonstrated higher alpha-glucosidase inhibitory activity. After ultrafiltration, the alpha-glucosidase inhibitory activity of different components was compared. The component (CSCPH-IV) with the highest activity was selected for further separation and purification. The peptides were purified using reverse-phase high-performance liquid chromatography (RP-HPLC) twice and identified as GHSLESIK, GLTSLDRYK and SPGYYDGR by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Subsequently, the preliminarily inhibitory effect of these peptides on alpha-glucosidase activity was demonstrated by the molecular docking model. These results showed that three novel peptides isolated from the hydrolysates of camellia seed cake have the potential to be used as anti-diabetic compounds for the development of functional foods.

引用

页码：138 / 147

页数：10

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