Palmitoyl-CoA synthetase on the external surface of isolated rat hepatocytes

被引：0

作者：

DeGroote, K ^{[1
]}

Velghe, G ^{[1
]}

Stals, HK ^{[1
]}

Baes, MI ^{[1
]}

Declercq, PE ^{[1
]}

机构：

[1] KATHOLIEKE UNIV LEUVEN,FAC PHARMACEUT SCI,DEPT CLIN CHEM,ONDERWIJS NAVORSING,B-3000 LOUVAIN,BELGIUM

来源：

CELL BIOCHEMISTRY AND FUNCTION | 1996年 / 14卷 / 04期

关键词：

long-chain acyl-CoA synthetase; plasma membrane; collagenase; (rat liver);

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

After incubating isolated rat hepatocytes with [1-C-14]palmitic acid, CoA and ATP (+MgCl2), a significant amount of [1-C-14]palmitoyl-CoA was found in the incubation medium. There was no correlation between its rate of synthesis and the degree of intactness of the cells. The results indicate that there is a long-chain fatty acyl-CoA synthetase active on the external surface of the hepatocyte plasma membrane. The activity of this enzyme was negligible in primary cultures of rat hepatocytes, suggesting that the exofacial long-chain acyl-CoA synthetase is an artifact of the collagenase perfusion technique used to prepare the hepatocytes.

引用

页码：277 / 281

页数：5

共 19 条

[1] ERYTHROCYTE-MEMBRANE REACYLATION IN JUVENILE NEURONAL CEROID-LIPOFUSCINOSIS - MEASUREMENT OF MEMBRANE-BOUND CARNITINE PALMITOYL TRANSFERASE, ACYL-COA SYNTHETASE, AND LYSOPHOSPHOLIPID - ACYL-COA ACYLTRANSFERASE ACTIVITIES [J].