Conjugated Linoleic Acid Negatively Regulates TR4 Activity in 3T3-L1 Adipocytes

被引：1
作者：
Choi, Hojung [1 ]
Kim, Eungseok [1 ]
机构：
[1] Chonnam Natl Univ, Dept Biol Sci, Kwangju 500757, South Korea
来源：
KOREAN JOURNAL FOR FOOD SCIENCE OF ANIMAL RESOURCES | 2011年 / 31卷 / 03期
关键词：
Conjugated linoleic acid; TR4; adipocytes; perilipin; ACTIVATED-RECEPTOR-GAMMA; DIRECT REPEAT 4; PPAR-GAMMA; ORPHAN RECEPTOR; INSULIN-RESISTANCE; EXPRESSION; OBESITY; SUPPRESSION; INHIBITION; MOUSE;
D O I：
10.5851/kosfa.2011.31.3.381
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Dietary conjugated linoleic acid (CLA) play key roles in lipid metabolism. Here, we investigated the effect of CLA on the transcriptional activity of TR4, an orphan nuclear receptor that plays an important role in lipid homeostasis. CLA increased TR4 gene mRNA level in 3T3-L1 adipocytes, but inhibited TR4 transcriptional activity in a dose-dependent manner. TR4 induced perilipin expression in 313-L1 adipocytes by activating perilipin promoter activity. In a gel shift assay, TR4 bound direct to the putative TR4 response element in the perilipin promoter. Interestingly, CLA reduced the interaction between TR4 and consensus DR1, a well-known TR4 binding site. Additionally, CLA inhibited TR4-induced perilipin promoter activity in a dose-dependent manner. Together, our results suggest that CLA may play a role in lipid homeostasis in adipocytes by functionally regulating TR4.
引用
收藏
页码：381 / 388
页数：8
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