Allele Intersection Analysis: A Novel Tool for Multi Locus Sequence Assignment in Multiply Infected Hosts

被引:20
作者
Arthofer, Wolfgang [1 ]
Riegler, Markus [2 ]
Schuler, Hannes [3 ]
Schneider, Daniela [5 ]
Moder, Karl [4 ]
Miller, Wolfgang J. [5 ]
Stauffer, Christian [3 ]
机构
[1] Univ Innsbruck, Inst Ecol, Mol Ecol Grp, A-6020 Innsbruck, Austria
[2] Univ Western Australia, Hawkesbury Inst Environm, Richmond, WA, Australia
[3] Univ Nat Resources & Life Sci, Inst Forest Entomol Forest Pathol & Forest Protec, Dept Forest & Soil Sci, Vienna, Austria
[4] Univ Nat Resources & Life Sci, Inst Appl Stat & Comp, Dept Landscape Spatial & Infrastruct Sci, Vienna, Austria
[5] Med Univ Vienna, Ctr Anat & Cell Biol, Vienna, Austria
基金
奥地利科学基金会;
关键词
RHAGOLETIS-CERASI DIPTERA; CHERRY FRUIT-FLY; WOLBACHIA-PIPIENTIS; CYTOPLASMIC INCOMPATIBILITY; DROSOPHILA-SIMULANS; RECOMBINATION; WSP; AMPLIFICATION; POPULATIONS; PHYLOGENY;
D O I
10.1371/journal.pone.0022198
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Wolbachia are wide-spread, endogenous alpha-Proteobacteria of arthropods and filarial nematodes. 15-75% of all insect species are infected with these endosymbionts that alter their host's reproduction to facilitate their spread. In recent years, many insect species infected with multiple Wolbachia strains have been identified. As the endosymbionts are not cultivable outside living cells, strain typing relies on molecular methods. A Multi Locus Sequence Typing (MLST) system was established for standardizing Wolbachia strain identification. However, MLST requires hosts to harbour individual and not multiple strains of supergroups without recombination. This study revisits the applicability of the current MLST protocols and introduces Allele Intersection Analysis (AIA) as a novel approach. AIA utilizes natural variations in infection patterns and allows correct strain assignment of MLST alleles in multiply infected host species without the need of artificial strain segregation. AIA identifies pairs of multiply infected individuals that share Wolbachia and differ in only one strain. In such pairs, the shared MLST sequences can be used to assign alleles to distinct strains. Furthermore, AIA is a powerful tool to detect recombination events. The underlying principle of AIA may easily be adopted for MLST approaches in other uncultivable bacterial genera that occur as multiple strain infections and the concept may find application in metagenomic high-throughput parallel sequencing projects.
引用
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页数:9
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