Sargahydroquinoic Acid Suppresses Hyperpigmentation by cAMP and ERK1/2-Mediated Downregulation of MITF in α-MSH-Stimulated B16F10 Cells

被引：12

作者：

Azam, Mohammed Shariful ^{[1
]}

Kim, Jae-Il ^{[1
]}

Choi, Chang Geun ^{[2
]}

Choi, Jinkyung ^{[1
]}

Lee, Bonggi ^{[1
]}

Kim, Hyeung-Rak ^{[1
]}

机构：

[1] Pukyong Natl Univ, Dept Food Sci & Nutr, 45 Yongso Ro, Busan 48513, South Korea

[2] Pukyong Natl Univ, Dept Ecol Engn, 45 Yongso Ro, Busan 48513, South Korea

来源：

FOODS | 2021年 / 10卷 / 10期

关键词：

cAMP; ERK; hyperpigmentation; melanin; MITF; sargahydroquinoic acid; INHIBITS MELANIN SYNTHESIS; SARGASSUM-SERRATIFOLIUM; ETHANOLIC EXTRACT; TRANSCRIPTION FACTOR; SARGAQUINOIC ACID; RICH FRACTION; MELANOGENESIS; PIGMENTATION; ACTIVATION; MELANOCYTES;

D O I：

10.3390/foods10102254

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Hyperpigmentation diseases of the skin require topical treatment with depigmenting agents. We investigated the hypopigmented mechanisms of sargahydroquinoic acid (SHQA) in alpha-melanocyte-stimulating hormone (alpha-MSH)-stimulated B16F10 cells. SHQA reduced cellular tyrosinase (TYR) activity and melanin content in a concentration-dependent manner and attenuated the expression of TYR and tyrosinase-related protein 1 (TRP1), along with their transcriptional regulator, microphthalmia-associated transcription factor (MITF). SHQA also suppressed alpha-MSH-induced cellular production of cyclic adenosine monophosphate (cAMP), which inhibited protein kinase A (PKA)-dependent cAMP-responsive element-binding protein (CREB) activation. Docking simulation data showed a potential binding affinity of SHQA to the regulatory subunit RII beta of PKA, which may also adversely affect PKA and CREB activation. Moreover, SHQA activated ERK1/2 signaling in B16F10 cells, stimulating the proteasomal degradation of MITF. These data suggest that SHQA ameliorated hyperpigmentation in alpha-MSH-stimulated B16F10 cells by downregulating MITF via PKA inactivation and ERK1/2 phosphorylation, indicating that SHQA is a potent therapeutic agent against skin hyperpigmentation disorders.

引用

页数：13

共 48 条

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