Determination of extended spectrum β-lactamases/AmpC β-lactamases and plasmid-mediated quinolone resistance in Escherichia coli isolates obtained from bovine carcasses in Mexico

被引:23
作者
Aguilar-Montes de Oca, Saul [1 ]
Talavera-Rojas, Martin [1 ]
Soriano-Vargas, Edgardo [1 ]
Barba-Leon, Jeannette [2 ]
Vazquez-Navarrete, Jesus [3 ]
机构
[1] Univ Autonoma Estado Mexico, Fac Med Vet & Zootecnia, Ctr Invest & Estudios Avanzados Salud Anim, Toluca 50200, State Of Mexico, Mexico
[2] Univ Guadalajara, Ctr Univ Ciencias Biol & Agr, Dept Salud Publ, Zapopan 45110, Jalisco, Mexico
[3] Inst Nacl Invest Forestales Agr & Pecuarias, Palo Alto 05110, DF, Mexico
关键词
Escherichia coli; Antibiotic resistance; beta-Lactams; Quinolones; ANTIMICROBIAL RESISTANCE; SALMONELLA-ENTERICA; MULTIPLEX PCR; UNITED-STATES; PREVALENCE; ANIMALS; GENES; FOOD; ENTEROBACTERIACEAE; SAMPLES;
D O I
10.1007/s11250-015-0818-3
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Food-borne bacterial infections have worldwide importance, and a great variety of antibiotic resistance mechanisms, mainly of the chromosome type, have rapidly developed. Antimicrobial resistance was determined in this study in terms of the presence of extended-spectrum beta-lactamases (ESBLs), plasmid AmpC beta-lactamases (pAmpC), and plasmid-mediated quinolone resistance (PMQR) from 155 Escherichia coli isolates obtained from bovine carcasses from two states in Mexico (states of Mexico and Jalisco). Isolates were challenged with beta-lactam antimicrobials (ampicillin, ceftazidime, and cefotaxime) and quinolones (nalidixic acid and ciprofloxacin). The presence of the bla (TEM), bla (SHV), bla (CTX-M), bla (OXA) , bla (CMY), bla (MOX), bla (LAT), bla (BIL), qnrA, qnrB, qnrS, aac(6')-Ib-cr, and qepA genes was examined by PCR. Clonal relationship was determined using pulsed-field gel electrophoresis (PFGE). The highest resistance was found to be to nalidixic acid (64 %), followed by ampicillin (32 %), ciprofloxacin (10 %), and ceftazidime and cefotaxime (both 1.3 %). bla (CMY) (n = 1), bla (TEM) (n = 24), qnrB (n = 9), and qnrS (n = 7) genes were detected. PFGE analysis showed that the majority of isolates had a different genotypic profile. To our knowledge, this is the first report of the presence of the qnrB, qnrS, and bla (CMY) genes in E. coli isolated from bovine meat in Mexico.
引用
收藏
页码:975 / 981
页数:7
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